Roy et al. (2018. J. Cell. Biol. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication forks. The sensitive and accurate SIRF methodology is suitable for multiparameter measurements in cell populations.
SIRFing the replication fork: Assessing protein interactions with nascent DNA / D. Branzei, M. Giannattasio. - In: THE JOURNAL OF CELL BIOLOGY. - ISSN 0021-9525. - 217:4(2018 Apr 28), pp. 1177-1179. [10.1083/jcb.201802083]
SIRFing the replication fork: Assessing protein interactions with nascent DNA
M. GiannattasioUltimo
2018
Abstract
Roy et al. (2018. J. Cell. Biol. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication forks. The sensitive and accurate SIRF methodology is suitable for multiparameter measurements in cell populations.File | Dimensione | Formato | |
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