The formation of lipid-DNA (CL-DNA) complexes called lipoplexes, proposed as DNA vectors in gene therapy, is obtained by adding DNA to a solution containing liposomes composed of cationic and neutral lipids. The structural and dynamic properties of such lipoplexes are determined by a coupling between the electrostatic interactions and the elastic parameters of the lipid mixture. An attempt to achieve a better understanding of the structure-dynamics relationship is reported herein. In particular, an elastic neutron scattering investigation of DOTAP-DOPC (dioleoyl trimethylammonium propane-dioleoyl phosphatidylcoline) complexed with DNA is described. Proton dynamics in this oriented CL-DNA lipoplex is found to be strongly dependent upon DNA concentration. Our results show that a substantial modification of the membrane dynamics is accompanied by the balancing of the total net charge inside the complex, together with the consequent displacement of interlayer water molecules.

Dynamic properties of an oriented lipid/DNA complex studied by neutron scattering / F. Natali, C. Castellano, D. Pozzi, A.C. Castellano. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - 88:2(2005 Feb), pp. 1081-1090.

Dynamic properties of an oriented lipid/DNA complex studied by neutron scattering

C. Castellano;
2005

Abstract

The formation of lipid-DNA (CL-DNA) complexes called lipoplexes, proposed as DNA vectors in gene therapy, is obtained by adding DNA to a solution containing liposomes composed of cationic and neutral lipids. The structural and dynamic properties of such lipoplexes are determined by a coupling between the electrostatic interactions and the elastic parameters of the lipid mixture. An attempt to achieve a better understanding of the structure-dynamics relationship is reported herein. In particular, an elastic neutron scattering investigation of DOTAP-DOPC (dioleoyl trimethylammonium propane-dioleoyl phosphatidylcoline) complexed with DNA is described. Proton dynamics in this oriented CL-DNA lipoplex is found to be strongly dependent upon DNA concentration. Our results show that a substantial modification of the membrane dynamics is accompanied by the balancing of the total net charge inside the complex, together with the consequent displacement of interlayer water molecules.
Myelin basic-protein; DNA complexes; molecular-dynamics; gene delivery; transfection efficiency; biological-membranes; computer-simulation; X-ray; condensation; lipofection
Settore CHIM/03 - Chimica Generale e Inorganica
Settore FIS/03 - Fisica della Materia
feb-2005
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/661075
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