In the present work curcumin loaded hyalurosomes were proposed as innovative systems for the treatment of rheumatoid arthritis. Vesicles were prepared using a one-step and environmentally friendly method. Aiming at finding the most suitable formulation in terms of size, surface charge and stability on storage, an extensive pre-formulation study was performed using different type and amount of phospholipids. Curcumin loaded vesicles prepared with 180 mg/ml of Phospholipon 90G (P90G) and immobilized with sodium hyaluronate (2 mg/ml) were selected because of their small size (189 nm), homogeneous dispersion (PI 0.24), negative charge (−35 mV), suitable ability to incorporate high amount of curcumin (E% ∼88%) and great stability on storage. The in vitro study using fibroblast-like synovial cells cultured in synovial fluid, demonstrated the ability of these vesicles to downregulate the production of anti-apoptotic proteins IAP1 and IAP2 and stimulate the production of IL-10, while the production of IL-6 and IL-15 and reactive oxygen species was reduced, confirming their suitability in counteracting pathogenesis of rheumatoid arthritis.
|Titolo:||Potential therapeutic effect of curcumin loaded hyalurosomes against inflammatory and oxidative processes involved in the pathogenesis of rheumatoid arthritis : the use of fibroblast-like synovial cells cultured in synovial fluid|
|Parole Chiave:||Curcumin; IL-6 and IL-15; In vitro inflammation; Oxidative stress; Phospholipid vesicles; Synoviocytes; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Arthritis, Rheumatoid; Cell Proliferation; Cells, Cultured; Curcumin; Drug Carriers; Fibroblasts; Humans; Hyaluronic Acid; Inflammation Mediators; Oxidative Stress; Phospholipids; Synovial Fluid; Synovial Membrane|
|Settore Scientifico Disciplinare:||Settore MED/33 - Malattie Apparato Locomotore|
|Data di pubblicazione:||2019|
|Digital Object Identifier (DOI):||10.1016/j.ejpb.2019.01.012|
|Appare nelle tipologie:||01 - Articolo su periodico|