We studied, by means of alternating current, the electrical properties of planar BLMs made of phosphatidylinositol (PI) or oxidized cholesterol, when incorporating mitochondrial porins (0 – 600 ng/ml). Porin induced an increase of membrane current with time. The maximum porin incorporation rate decreased lowering porin concentration (C). The steady current increased with C. For a same C the incorporation kinetics were faster and higher steady levels were reached in oxidized cholesterol than in PI membranes. The highest values of conductance reached in PI membranes (also with higher C) were always lower than the lowest values obtained with oxidized cholesterol membranes. The applied voltage affected the kinetics and the channel formation. The results show the possibility to modulate the electrical conductance of BLMs by choosing properly the conditions under which to incorporate the protein molecules

MODULATION OF ELECTRICAL CONDUCTANCE IN PLANAR LIPID BILAYER MEMBRANES (BLMs) / G. Monticelli, S. Micelli, E. Gallucci, A. Aceti. ((Intervento presentato al 11. convegno INTERNATIONAL BIOPHYSICS CONGRESS tenutosi a Budapest nel 1993.

MODULATION OF ELECTRICAL CONDUCTANCE IN PLANAR LIPID BILAYER MEMBRANES (BLMs)

G. Monticelli
Primo
;
1993

Abstract

We studied, by means of alternating current, the electrical properties of planar BLMs made of phosphatidylinositol (PI) or oxidized cholesterol, when incorporating mitochondrial porins (0 – 600 ng/ml). Porin induced an increase of membrane current with time. The maximum porin incorporation rate decreased lowering porin concentration (C). The steady current increased with C. For a same C the incorporation kinetics were faster and higher steady levels were reached in oxidized cholesterol than in PI membranes. The highest values of conductance reached in PI membranes (also with higher C) were always lower than the lowest values obtained with oxidized cholesterol membranes. The applied voltage affected the kinetics and the channel formation. The results show the possibility to modulate the electrical conductance of BLMs by choosing properly the conditions under which to incorporate the protein molecules
27-lug-1993
phosphatidylinositol ; oxidized cholesterol ; lipid bilayer membranes ; BLM ; mitochondrial porin ; channel formation ; membrane conductance
Settore BIO/09 - Fisiologia
International Union for Pure and Applied Biophysics
Hungarian Biophysical Society
Hungarian Academy of Sciences
MODULATION OF ELECTRICAL CONDUCTANCE IN PLANAR LIPID BILAYER MEMBRANES (BLMs) / G. Monticelli, S. Micelli, E. Gallucci, A. Aceti. ((Intervento presentato al 11. convegno INTERNATIONAL BIOPHYSICS CONGRESS tenutosi a Budapest nel 1993.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/66065
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