Background: Activated intermediates of 4-aminobiphenyl (4-ABP) are able to covalently interact with DNA to form adducts. There ir a large body of evidence indicating that carcinogen-DNA adduct formation can be one of the cancer initiating mechanisms. Materials and Methods. (4-ABP)-induced DNA damage in association with p53 overexpression and mutations were evaluated in specimens of urothelial bladder cancers from 106 patients. Results: 4-ABP-DNA adduct levels resulted higher in smokers compared to non smokers, with a borderline statistical value. p53 nuclear overexpression was related to tumor grading, while no significant correlation with stage, 4-ABP-DNA adducts, smoking habit, and disease recurrence could be observed. Concerning molecular analysis, p53 point mutations were found in 17 of 106 cases (16%) and mutational pattern was significantly associated both with higher grade and stage, but no correlation was found with disease recurrence. Conclusions: These results suggest that other sources, in addition to tobacco smoke, may contribute to 4-ABP-DNA adducts formation in bladder tissue and that p53 expression/mutation cannot be considered a prognostic factor in bladder cancer.
Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma / G. Romano, L. Garagnani, A. Boninsegna, P. Ferrari, G. Flamini, C. De Gaetani, A. Sgambato, F. Giovanni, G. Curigliano, G. Ferretti, A. Cittadini, G. Trentini. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 19:5C(1999), pp. 4571-4576.
|Titolo:||Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma|
|Parole Chiave:||4-ABP-DNA adducts; p53 overexpression/mutations; cigarette smoke; bladder cancer; molecular epidemiology|
|Settore Scientifico Disciplinare:||Settore MED/06 - Oncologia Medica|
|Data di pubblicazione:||1999|
|Appare nelle tipologie:||01 - Articolo su periodico|