Tumors driven by activation of the transcription factor MYC generally show oncogene addiction. However, the gene expression programs that depend upon sustained MYC activity remain unknown. In this study, we employed a mouse model of liver carcinoma driven by a reversible tet-MYC transgene, combined with chromatin immunoprecipitation and gene expression profiling to identify MYC-dependent regulatory events. As previously reported, MYC-expressing mice exhibited hepatoblastoma- and hepatocellular carcinoma-like tumors, which regressed when MYC expression was suppressed. We further show that cellular transformation, and thus initiation of liver tumorigenesis, were impaired in mice harboring a MYC mutant unable to associate with the corepressor protein MIZ1 (ZBTB17). Notably, switching off the oncogene in advanced carcinomas revealed that MYC was required for the continuous activation and repression of distinct sets of genes, constituting no more than half of all genes deregulated during tumor progression and an even smaller subset of all MYC-bound genes. Altogether, our data provide the first detailed analysis of a MYC-dependent transcriptional program in a fully developed carcinoma and offer a guide to identifying the critical effectors contributing to MYC-driven tumor maintenance.

Identification of MYC-dependent transcriptional programs in oncogene-addicted liver tumors / T.R. Kress, P. Pellanda, L. Pellegrinet, V. Bianchi, P. Nicoli, M. Doni, C. Recordati, S. Bianchi, L. Rotta, T. Capra, M. Rava, A. Verrecchia, E. Radaelli, T.D. Littlewood, G.I. Evan, B. Amati. - In: CANCER RESEARCH. - ISSN 0008-5472. - 76:12(2016 Jun 15), pp. 3463-3472. [10.1158/0008-5472.CAN-16-0316]

Identification of MYC-dependent transcriptional programs in oncogene-addicted liver tumors

P. Pellanda;C. Recordati;T. Capra;M. Rava;E. Radaelli;
2016

Abstract

Tumors driven by activation of the transcription factor MYC generally show oncogene addiction. However, the gene expression programs that depend upon sustained MYC activity remain unknown. In this study, we employed a mouse model of liver carcinoma driven by a reversible tet-MYC transgene, combined with chromatin immunoprecipitation and gene expression profiling to identify MYC-dependent regulatory events. As previously reported, MYC-expressing mice exhibited hepatoblastoma- and hepatocellular carcinoma-like tumors, which regressed when MYC expression was suppressed. We further show that cellular transformation, and thus initiation of liver tumorigenesis, were impaired in mice harboring a MYC mutant unable to associate with the corepressor protein MIZ1 (ZBTB17). Notably, switching off the oncogene in advanced carcinomas revealed that MYC was required for the continuous activation and repression of distinct sets of genes, constituting no more than half of all genes deregulated during tumor progression and an even smaller subset of all MYC-bound genes. Altogether, our data provide the first detailed analysis of a MYC-dependent transcriptional program in a fully developed carcinoma and offer a guide to identifying the critical effectors contributing to MYC-driven tumor maintenance.
Animals; Carcinoma, Hepatocellular; Cells, Cultured; Gene Expression Regulation, Neoplastic; Humans; Liver Neoplasms; Mice; Promoter Regions, Genetic; Proto-Oncogene Proteins c-myc; Oncogenes; Transcription, Genetic
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/658214
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