During development, ribosome biogenesis and translation reach peak activities, due to impetuous cell proliferation. Current models predict that protein synthesis elevation is controlled by transcription factors and signalling pathways. Developmental models addressing translation factors overexpression effects are lacking. Eukaryotic Initiation Factor 6 (eIF6) is necessary for ribosome biogenesis and efficient translation. eIF6 is a single gene, conserved from yeasts to mammals, suggesting a tight regulation need. We generated a Drosophila melanogaster model of eIF6 upregulation, leading to a boost in general translation and the shut-down of the ecdysone biosynthetic pathway. Indeed, translation modulation in S2 cells showed that translational rate and ecdysone biosynthesis are inversely correlated. In vivo, eIF6-driven alterations delayed Programmed Cell Death (PCD), resulting in aberrant phenotypes, partially rescued by ecdysone administration. Our data show that eIF6 triggers a translation program with far-reaching effects on metabolism and development, stressing the driving and central role of translation.

Modulating eIF6 levels unveils the role of translation in ecdysone biosynthesis during Drosophila development / A. Russo, G. Gatti, R. Alfieri, E. Pesce, K. Soanes, S. Ricciardi, M. Mancino, C. Cheroni, T. Vaccari, S. Biffo, P. Calamita. - In: DEVELOPMENTAL BIOLOGY. - ISSN 0012-1606. - 445:1(2019 Nov), pp. 100-111. [10.1016/j.ydbio.2019.05.013]

Modulating eIF6 levels unveils the role of translation in ecdysone biosynthesis during Drosophila development

Pesce, Elisa;Ricciardi, Sara;Cheroni, Cristina;Vaccari, Thomas;Biffo, Stefano;Calamita, Piera
2019-11

Abstract

During development, ribosome biogenesis and translation reach peak activities, due to impetuous cell proliferation. Current models predict that protein synthesis elevation is controlled by transcription factors and signalling pathways. Developmental models addressing translation factors overexpression effects are lacking. Eukaryotic Initiation Factor 6 (eIF6) is necessary for ribosome biogenesis and efficient translation. eIF6 is a single gene, conserved from yeasts to mammals, suggesting a tight regulation need. We generated a Drosophila melanogaster model of eIF6 upregulation, leading to a boost in general translation and the shut-down of the ecdysone biosynthetic pathway. Indeed, translation modulation in S2 cells showed that translational rate and ecdysone biosynthesis are inversely correlated. In vivo, eIF6-driven alterations delayed Programmed Cell Death (PCD), resulting in aberrant phenotypes, partially rescued by ecdysone administration. Our data show that eIF6 triggers a translation program with far-reaching effects on metabolism and development, stressing the driving and central role of translation.
20HE; Drosophila development; Ecdysone; Translation; eIF6
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
Noncoding and Translational Modulation of Gene Expression and Epigenetic Changes
5-lug-2019
DEVELOPMENTAL BIOLOGY
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0012160618307966-main.pdf

non disponibili

Tipologia: Publisher's version/PDF
Dimensione 3.13 MB
Formato Adobe PDF
3.13 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Modulating eIF6 levels unveils the role of translation in ecdysone biosynthesis during Drosophila development.pdf

accesso aperto

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 1 MB
Formato Adobe PDF
1 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/657574
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact