During development, ribosome biogenesis and translation reach peak activities, due to impetuous cell proliferation. Current models predict that protein synthesis elevation is controlled by transcription factors and signalling pathways. Developmental models addressing translation factors overexpression effects are lacking. Eukaryotic Initiation Factor 6 (eIF6) is necessary for ribosome biogenesis and efficient translation. eIF6 is a single gene, conserved from yeasts to mammals, suggesting a tight regulation need. We generated a Drosophila melanogaster model of eIF6 upregulation, leading to a boost in general translation and the shut-down of the ecdysone biosynthetic pathway. Indeed, translation modulation in S2 cells showed that translational rate and ecdysone biosynthesis are inversely correlated. In vivo, eIF6-driven alterations delayed Programmed Cell Death (PCD), resulting in aberrant phenotypes, partially rescued by ecdysone administration. Our data show that eIF6 triggers a translation program with far-reaching effects on metabolism and development, stressing the driving and central role of translation.
Modulating eIF6 levels unveils the role of translation in ecdysone biosynthesis during Drosophila development / A. Russo, G. Gatti, R. Alfieri, E. Pesce, K. Soanes, S. Ricciardi, M. Mancino, C. Cheroni, T. Vaccari, S. Biffo, P. Calamita. - In: DEVELOPMENTAL BIOLOGY. - ISSN 0012-1606. - 445:1(2019 Nov), pp. 100-111.
Titolo: | Modulating eIF6 levels unveils the role of translation in ecdysone biosynthesis during Drosophila development |
Autori: | CALAMITA, PIERA (Corresponding) |
Parole Chiave: | 20HE; Drosophila development; Ecdysone; Translation; eIF6 |
Settore Scientifico Disciplinare: | Settore BIO/06 - Anatomia Comparata e Citologia Settore BIO/11 - Biologia Molecolare Settore BIO/13 - Biologia Applicata |
Progetto: | Noncoding and Translational Modulation of Gene Expression and Epigenetic Changes |
Data di pubblicazione: | nov-2019 |
Rivista: | |
Tipologia: | Article (author) |
Data ahead of print / Data di stampa: | 5-lug-2019 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.ydbio.2019.05.013 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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