Plants develop in a microbe-rich environment and must interact with a plethora of microorganisms, both pathogenic and beneficial. Indeed, such is the case of Pseudomonas, and its model organisms P. fluorescens and P. syringae, a bacterial genus that has received particular attention because of its beneficial effect on plants and its pathogenic strains. The present study aims to compare plant-beneficial and pathogenic strains belonging to the P. syringae species to get new insights into the distinction between the two types of plant-microbe interactions. In assays carried out under greenhouse conditions, P. syringae pv. syringae strain 260-02 was shown to promote plant-growth and to exert biocontrol of P. syringae pv. tomato strain DC3000, against the Botrytis cinerea fungus and the Cymbidium Ringspot Virus. This P. syringae strain also had a distinct volatile emission profile, as well as a different plant-colonization pattern, visualized by confocal microscopy and gfp labeled strains, compared to strain DC3000. Despite the different behavior, the P. syringae strain 260-02 showed great similarity to pathogenic strains at a genomic level. However, genome analyses highlighted a few differences that form the basis for the following hypotheses regarding strain 260-02. P. syringae strain 260-02: (i) possesses nonfunctional virulence genes, like the mangotoxin-producing operon Mbo; (ii) has different regulation pathways, suggested by the difference in the autoinducer system and the lack of a virulence activator gene; (iii) has genes encoding DNA methylases different from those found in other P. syringae strains, suggested by the presence of horizontal-gene-transfer-obtained methylases that could affect gene expression.
Not Just a Pathogen? : Description of a Plant-Beneficial Pseudomonas syringae Strain / A. Passera, S. Compant, P. Casati, M.G. Maturo, G. Battelli, F. Quaglino, L. Antonielli, D. Salerno, M. Brasca, S.L. Toffolatti, F. Mantegazza, M. Delledonne, B. Mitter. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 10(2019 Jun 21), pp. 1409.1-1409.21.
|Titolo:||Not Just a Pathogen? : Description of a Plant-Beneficial Pseudomonas syringae Strain|
PASSERA, ALESSANDRO (Primo) (Corresponding)
|Parole Chiave:||Pseudomonas syringae; biocontrol; pangenome analysis; confocal microscopy; Botrytis cinerea|
|Settore Scientifico Disciplinare:||Settore AGR/12 - Patologia Vegetale|
|Data di pubblicazione:||21-giu-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3389/fmicb.2019.01409|
|Appare nelle tipologie:||01 - Articolo su periodico|