A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
Semisynthesis of dimeric proteins by expressed protein ligation / B. Ziaco, S. Pensato, L.D. D'Andrea, E. Benedetti, A. Romanelli. - In: ORGANIC LETTERS. - ISSN 1523-7060. - 10:10(2008), pp. 1955-1958.
Semisynthesis of dimeric proteins by expressed protein ligation
A. Romanelli
2008
Abstract
A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
File in questo prodotto:
| File | Dimensione | Formato | |
|---|---|---|---|
|
OL08_SI.pdf
accesso aperto
Descrizione: published paper
Tipologia:
Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione
119.33 kB
Formato
Adobe PDF
|
119.33 kB | Adobe PDF | Visualizza/Apri |
|
ol800457g.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
380.22 kB
Formato
Adobe PDF
|
380.22 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
