The ability of PNA to interact with DNA double stranded has been recently investigated. In a decoy approach these interactions are of great importance as may lead to inhibition of interactions of DNA sequences to specific transcription factors and may be employed as a strategy for the inhibition of gene transcription alternative to the antisense strategy (targeting transcription factors mRNAs) and the transcription factor decoy approach (targeting transcription factors). We explored the ability of PNA and PNAs with modified monomers to bind to DNA and to interfere in the formation of DNA/transcription factor complex. We report a procedure for the synthesis of Fmoc-gamma-hydroxymetyl PNA, the synthesis and CD analysis of PNA oligomers containing the modified monomer in different positions and EMSA assays to test the: (a) binding to double stranded DNA and (b) inhibition of DNA-protein interactions.
γ-Hydroxymethyl PNAs : synthesis, interaction with DNA and inhibition of protein/DNA interactions / S. Pensato, M. Saviano, N. Bianchi, M. Borgatti, E. Fabbri, R. Gambari, A. Romanelli. - In: BIOORGANIC CHEMISTRY. - ISSN 0045-2068. - 38:5(2010), pp. 196-201.
|Titolo:||γ-Hydroxymethyl PNAs : synthesis, interaction with DNA and inhibition of protein/DNA interactions|
ROMANELLI, ALESSANDRA (Corresponding)
|Parole Chiave:||Peptide Nucleic Acid, synthesis, Circular Dicroism|
|Settore Scientifico Disciplinare:||Settore CHIM/03 - Chimica Generale e Inorganica|
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.bioorg.2010.06.002|
|Appare nelle tipologie:||01 - Articolo su periodico|