An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%.
Development of an efficient and low-cost protocol for the manual PNA synthesis by Fmoc chemistry / C. Avitabile, L. Moggio, L.D. D'Andrea, C. Pedone, A. Romanelli. - In: TETRAHEDRON LETTERS. - ISSN 0040-4039. - 51:29(2010), pp. 3716-3718.
Development of an efficient and low-cost protocol for the manual PNA synthesis by Fmoc chemistry
A. Romanelli
2010
Abstract
An efficient and low-cost protocol for the manual synthesis of Peptide Nucleic Acids is reported here. The protocol relies on coupling reactions carried out with 2.5 equiv of PNA monomers activated with HOBT/HBTU, in the presence of pyridine/NMM. The protocol has been tested on four PNA oligomers with a length ranging from 9 to 12 bases and a purine content up to 67%.
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