DNA damage checkpoints are surveillance mechanisms that monitor the integrity of the genome. Nucleotide excision repair (NER) is a DNA repair mechanism that cells use to remove UV-induced DNA lesions. Previous publication from our laboratory demonstrated that recognition and processing of UV-induced damage by NER is required for proper activation of checkpoint through interactions between NER proteins and checkpoint factors in yeast and human primary fibroblasts. From a two hybrid screening in yeast exonuclease 1 (Exo1) was identified as a 9-1-1 complex interactor. Exo1 is a 5’-3’ exonuclease and 5'-flap-endonuclease with many different roles in DNA metabolism such as meiotic and mitotic recombination, mismatch repair and telomere processing. Characterization of an exo1 yeast deleted strain has shown that this protein is involved in the early steps of UV-induced DNA damage checkpoint. In human cells EXO1 is present as two isoforms named hEXO1a and hEXO1b genetarated by alternative splicing. We are analyzing the role of EXO1 in checkpoint activation in response to UV-C damage in human cells: using siRNA against both a and b isoform of hEXO1 in G1 cells we were able to observe a defect in Chk1 and p53 phosphorylation induced by UV-C irradiation.

Human exonuclease 1 role in response to UV irradiation / S. Sertic, M. Giannattasio, F. Puddu, M. Marini, G. Rotondo, M. Muzi Falconi, P. Plevani. ((Intervento presentato al convegno Workshop for young scientists, 3rd EU-IP DNA REPAIR tenutosi a Taormina nel 2009.

Human exonuclease 1 role in response to UV irradiation

S. Sertic;M. Giannattasio;F. Puddu;M. Marini;G. Rotondo;M. Muzi Falconi;P. Plevani
2009

Abstract

DNA damage checkpoints are surveillance mechanisms that monitor the integrity of the genome. Nucleotide excision repair (NER) is a DNA repair mechanism that cells use to remove UV-induced DNA lesions. Previous publication from our laboratory demonstrated that recognition and processing of UV-induced damage by NER is required for proper activation of checkpoint through interactions between NER proteins and checkpoint factors in yeast and human primary fibroblasts. From a two hybrid screening in yeast exonuclease 1 (Exo1) was identified as a 9-1-1 complex interactor. Exo1 is a 5’-3’ exonuclease and 5'-flap-endonuclease with many different roles in DNA metabolism such as meiotic and mitotic recombination, mismatch repair and telomere processing. Characterization of an exo1 yeast deleted strain has shown that this protein is involved in the early steps of UV-induced DNA damage checkpoint. In human cells EXO1 is present as two isoforms named hEXO1a and hEXO1b genetarated by alternative splicing. We are analyzing the role of EXO1 in checkpoint activation in response to UV-C damage in human cells: using siRNA against both a and b isoform of hEXO1 in G1 cells we were able to observe a defect in Chk1 and p53 phosphorylation induced by UV-C irradiation.
No
English
feb-2009
Settore BIO/11 - Biologia Molecolare
Poster
Intervento inviato
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Pubblicazione scientifica
Workshop for young scientists, 3rd EU-IP DNA REPAIR
Taormina
2009
Convegno internazionale
S. Sertic, M. Giannattasio, F. Puddu, M. Marini, G. Rotondo, M. Muzi Falconi, P. Plevani
Human exonuclease 1 role in response to UV irradiation / S. Sertic, M. Giannattasio, F. Puddu, M. Marini, G. Rotondo, M. Muzi Falconi, P. Plevani. ((Intervento presentato al convegno Workshop for young scientists, 3rd EU-IP DNA REPAIR tenutosi a Taormina nel 2009.
Prodotti della ricerca::14 - Intervento a convegno non pubblicato
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/653221
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