Peripheral blood mononuclear cells of multiple sclerosis (MS) patients were stimulated with myelin basic protein (MBP) together with anti-CD28 monoclonal antibody and staphylococcal enterotoxin B to optimize cytokine production by antigen-specific cells. Type 1 (IL-2, IL-12, IFNγ) and pro-inflammatory (TNFα, IL-1β, IL-6) cytokines were augmented in CD4+, CD8+, and CD14+ cells of acute MS patients and of patients undergoing disease reactivation. These cytokines were reduced in IFNβ-treated and in stable MS patients; type 2 cytokines (IL-4, IL-10) were increased in these patients. Similar immune profiles are seen in MS patients in whom remission is naturally or pharmacologically (IFNβ) achieved. Cytokine alterations are particularly evident in CD14+ cells, underlying their critical role in the modulation of the immune response.
Single-cell analysis of cytokine production shows different immune profiles in multiple sclerosis patients with active or quiescent disease / M. Clerici, M. Saresella, D. Trabattoni, L. Speciale, S. Fossati, S. Ruzzante, R. Cavaretta, M. Filippi, D. Caputo, P. Ferrante. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 121:1-2(2001 Dec), pp. 88-101.
|Titolo:||Single-cell analysis of cytokine production shows different immune profiles in multiple sclerosis patients with active or quiescent disease|
CLERICI, MARIO SALVATORE (Primo)
FERRANTE, PASQUALE (Ultimo)
|Parole Chiave:||Cell-mediated immunity; Cytokines; Interferon beta; Multiple sclerosis; Th1/Th2|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Data di pubblicazione:||dic-2001|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0165-5728(01)00431-3|
|Appare nelle tipologie:||01 - Articolo su periodico|