Background: Accurate quantification of total HIV-DNA and residual-viremia by sensitive assays is extremely useful to optimize monitoring of ART-treated patients. Objectives: To evaluate the performances of two ddPCR-based assays for HIV-DNA and residual-viremia quantification, and the correlations of pre-ART HIV-DNA with plasma HIV-RNA, CD4 + T, CD4/CD8 and virological-success (VS) during first-line ART. Study design: Plasma HIV-RNA, total HIV-DNA, CD4 + T, CD4/CD8 were evaluated at baseline of ART, at VS (viral-load <50copies/ml), and at 6 months after VS (6moVS) in 57 newly-diagnosed HIV-1 infected patients, receiving first-line modern ART. HIV-DNA (log10 copies/106CD4 + T) and residual-viremia (copies/ml) were measured with in-house ddPCR assays. Correlations were assessed by Spearman and Jonckheere-Terpstra tests. Results: HIV-DNA and residual-viremia assays showed a good linear trend between the expected and obtained values (R2 = 0.9913 and 0.9945); lower limits of detection were 32 copies/106CD4 + T and 2 copies/ml, respectively. At baseline, median (IQR) plasma HIV-RNA and HIV-DNA were 4.88(4.28–5.36)log10 copies/ml and 4.00(3.36–4.51) log10 copies/106CD4 + T cells. Residual-viremia was 8(2–26) and 4(2–12) copies/ml at VS and 6moVS. Pre-ART HIV-DNA positively correlated with plasma HIV-RNA at BL (Rho = 0.708, p < 0.001), and with residual-viremia at VS (Rho:0.383,p = 0.002). Notably, higher HIV-DNA correlated with longer time to achieve VS (median[IQR],weeks: 17.8[12.3–29.0] for HIV-DNA ≥4.5 vs. 7.4[4.1–8.7] for HIV-DNA<4.5, p < 0.001). Furthermore, pre-ART HIV-DNA negatively correlated with CD4 + T and CD4/CD8 at baseline, VS and 6moVS. Conclusions: Our results support the adoption of ddPCR-based assays for both HIV-DNA and residual-viremia quantifications and corroborate that pre-ART HIV-DNA is an excellent indicator in predicting viroimmunological response and VS in patients starting ART.
Quantification of HIV-DNA and residual viremia in patients starting ART by droplet digital PCR: Their dynamic decay and correlations with immunological parameters and virological success / C. Alteri, R. Scutari, C. Stingone, G. Maffongelli, M. Brugneti, F. Falasca, S. Martini, A. Bertoli, O. Turriziani, L. Sarmati, N. Coppola, M. Andreoni, M.M. Santoro, C.F. Perno, F. Ceccherini-Silberstein, V. Svicher. - In: JOURNAL OF CLINICAL VIROLOGY. - ISSN 1386-6532. - 117(2019 Aug), pp. 61-67.
|Titolo:||Quantification of HIV-DNA and residual viremia in patients starting ART by droplet digital PCR: Their dynamic decay and correlations with immunological parameters and virological success|
ALTERI, CLAUDIA (Primo) (Corresponding)
|Parole Chiave:||Antiretroviral treatment; ddPCR; HIV-1; HIV-DNA; HIV-reservoir; Residual viremia|
|Settore Scientifico Disciplinare:||Settore MED/07 - Microbiologia e Microbiologia Clinica|
|Data di pubblicazione:||ago-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jcv.2019.06.004|
|Appare nelle tipologie:||01 - Articolo su periodico|