The possibility of identifying or tracing the primary product, mainly the origin breed, by the use of biologic markers, is an important goal for national goat cheese. In this field great interest is paid to milk protein genetic polymorphism. In order to perform an investigation on the possibility of tracing the source milk in goat dairy products, a study was carried out on the use of molecular techniques for the analysis of goat cheese. Fourteen cheeses with different origin were analysed. A good DNA was obtained by the use of a commercial kit and it was amplified for the analysis of the genetic polymorphism at αs1-Cn, αs2-Cn and k-Cn loci and successfully submitted to different molecular techniques (PCR-SSCP, PCR-RFLP, AS-PCR). The results at k-Cn locus differentiated between goat breeds showing typical breed alleles. The analysis will aim both to detect frauds and to identify particular alleles characterising local breeds interesting for the safeguard of the Italian animal resources and the national typical products
Un approccio molecolare nello studio della Tracciabilità dei prodotti caseari caprini / G. Ceriotti, S. Chessa, G. Bramante, P. Bolla, E. Pieragostini, A. Caroli. - In: SCIENZA E TECNICA LATTIERO-CASEARIA. - ISSN 0390-6361. - 55:(2004), pp. 251-262.
Un approccio molecolare nello studio della Tracciabilità dei prodotti caseari caprini
G. CeriottiPrimo
;S. ChessaSecondo
;P. Bolla;
2004
Abstract
The possibility of identifying or tracing the primary product, mainly the origin breed, by the use of biologic markers, is an important goal for national goat cheese. In this field great interest is paid to milk protein genetic polymorphism. In order to perform an investigation on the possibility of tracing the source milk in goat dairy products, a study was carried out on the use of molecular techniques for the analysis of goat cheese. Fourteen cheeses with different origin were analysed. A good DNA was obtained by the use of a commercial kit and it was amplified for the analysis of the genetic polymorphism at αs1-Cn, αs2-Cn and k-Cn loci and successfully submitted to different molecular techniques (PCR-SSCP, PCR-RFLP, AS-PCR). The results at k-Cn locus differentiated between goat breeds showing typical breed alleles. The analysis will aim both to detect frauds and to identify particular alleles characterising local breeds interesting for the safeguard of the Italian animal resources and the national typical productsPubblicazioni consigliate
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