Objective: Oxidative stress has been related both to the aging process and to several pathologies, like cancer and cardiovascular disease. Up to now, no ideal biomaxker for oxidative stress is available. This study was performed to identify a comprehensive index of oxidative status (OXISCORE)reflecting both injuries and antioxidant defences in vivo. Methods: We enrolled and stratified by age and gender, 87 healthy subjects (59% males; age 24-77) and 20 CAD patients (65% males; age 47-81). Plasma free and total malondialdehyde (F- and T-MDA), glutathione disulphide/reduced forms (GSSG/GSH) and urine isoprostane (iPF2alpha-III)levels were measured as markers of oxidative damage, and computed in the damage score (DS). Plasma glutathione, alpha- and gamma-tocopherol (TH) levels, and individual antioxidant capacity (IAC), were measured as indexes of antioxidant defence, and computed in the protection score (PS). OXISCORE was computed by subtracting PS from DS. Results: In healthy subjects, only MDA was associated with age; only GSH, GSSG/GSH ratio and alpha-TH correlated with gender. OXISCORE,was positively associated with both age (p=0.007) and male gender (p=0.01). Interestingly, OXISCORE was significantly higher in CAD subjects even after adjusting for age and gender (p=0.0001). Conclusions: No single parameter fully reflects the redox status. By representing both antioxidant and oxidative systems, the OXISCORE provides a comprehensive index of oxidative stress related to age, gender and CAD status. Funding: Grant from Italian Ministry of Health
A proposed new comprehensive index of oxidative stress: Oxiscore / F. Veglia, G. Cighetti, L. Zingaro, E. Tremoli, V.M. Cavalca. - In: ATHEROSCLEROSIS SUPPLEMENTS. - ISSN 1567-5688. - 7:3(2006), pp. 294-294(Tu-P10:498). ((Intervento presentato al 14. convegno International Symposium on Atherosclerosis tenutosi a Roma, Italy nel 2006 [10.1016/S1567-5688(06)81198-0].
A proposed new comprehensive index of oxidative stress: Oxiscore
G. CighettiSecondo
;E. TremoliPenultimo
;V.M. CavalcaUltimo
2006
Abstract
Objective: Oxidative stress has been related both to the aging process and to several pathologies, like cancer and cardiovascular disease. Up to now, no ideal biomaxker for oxidative stress is available. This study was performed to identify a comprehensive index of oxidative status (OXISCORE)reflecting both injuries and antioxidant defences in vivo. Methods: We enrolled and stratified by age and gender, 87 healthy subjects (59% males; age 24-77) and 20 CAD patients (65% males; age 47-81). Plasma free and total malondialdehyde (F- and T-MDA), glutathione disulphide/reduced forms (GSSG/GSH) and urine isoprostane (iPF2alpha-III)levels were measured as markers of oxidative damage, and computed in the damage score (DS). Plasma glutathione, alpha- and gamma-tocopherol (TH) levels, and individual antioxidant capacity (IAC), were measured as indexes of antioxidant defence, and computed in the protection score (PS). OXISCORE was computed by subtracting PS from DS. Results: In healthy subjects, only MDA was associated with age; only GSH, GSSG/GSH ratio and alpha-TH correlated with gender. OXISCORE,was positively associated with both age (p=0.007) and male gender (p=0.01). Interestingly, OXISCORE was significantly higher in CAD subjects even after adjusting for age and gender (p=0.0001). Conclusions: No single parameter fully reflects the redox status. By representing both antioxidant and oxidative systems, the OXISCORE provides a comprehensive index of oxidative stress related to age, gender and CAD status. Funding: Grant from Italian Ministry of Health
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