Volatile terpenes (VTs) content in tree-ring resin, in response to natural infection by Heterobasidion spp. in asymptomatic mature Norway spruce (Picea abies) trees was investigated. Twenty-three randomly selected mature trees were sampled in a stand in the Western Italian Alps by extracting cores using an increment borer. Based on fungal isolations from cores and molecular typing using taxon-specific competitive-priming (TSCP)-polymerase chain reaction, 12 out of the 23 trees were identified as infected by Heterobasidion parviporum. Tree-ring growth patterns and VT content in tree rings were determined. Analysis of VT content was performed by means of gas chromatography mass spectrometry on a subset of trees. Results show slightly but not significantly lower tree-ring width in infected compared with non-infected trees in the past two decades. Total concentrations of sesquiterpenes (SQTs) and relative proportions of -pinene, -pinene and longifolene were significantly greater in infected trees; while relative proportions of camphene, 3-carene, -cymene, sesquiterpene 15.90 and -farnesene were significantly lower. This is the first study showing that VTs in tree-ring resin may indicate infection of trees by a fungal forest pathogen, even when trees are mostly asymptomatic.
Tree-ring volatile terpenes show potential to indicate fungal infection in asymptomatic mature Norway spruce trees in the Alps / L.C. Vezzola, M. Michelozzi, L. Calamai, P. Gonthier, L. Giordano, P. Cherubini, M. Pelfini. - In: FORESTRY. - ISSN 0015-752X. - 92:2(2019 Apr), pp. 149-156. [10.1093/forestry/cpy041]
Tree-ring volatile terpenes show potential to indicate fungal infection in asymptomatic mature Norway spruce trees in the Alps
L.C. Vezzola
;M. Pelfini
2019
Abstract
Volatile terpenes (VTs) content in tree-ring resin, in response to natural infection by Heterobasidion spp. in asymptomatic mature Norway spruce (Picea abies) trees was investigated. Twenty-three randomly selected mature trees were sampled in a stand in the Western Italian Alps by extracting cores using an increment borer. Based on fungal isolations from cores and molecular typing using taxon-specific competitive-priming (TSCP)-polymerase chain reaction, 12 out of the 23 trees were identified as infected by Heterobasidion parviporum. Tree-ring growth patterns and VT content in tree rings were determined. Analysis of VT content was performed by means of gas chromatography mass spectrometry on a subset of trees. Results show slightly but not significantly lower tree-ring width in infected compared with non-infected trees in the past two decades. Total concentrations of sesquiterpenes (SQTs) and relative proportions of -pinene, -pinene and longifolene were significantly greater in infected trees; while relative proportions of camphene, 3-carene, -cymene, sesquiterpene 15.90 and -farnesene were significantly lower. This is the first study showing that VTs in tree-ring resin may indicate infection of trees by a fungal forest pathogen, even when trees are mostly asymptomatic.File | Dimensione | Formato | |
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