At least two distinct modalities of cell death, apoptosis and necrosis, can be distinguished based on differences in morphological, biochemical, and molecular changes of dying cells [1, 2]. Cell death is involved both in physiological and pathological conditions of the skeleton: for example, apoptosis is a crucial event during limb development. Therefore, detection of cell death by using a simple stain is a powerful tool to study molecular and cellular mechanisms of skeletal development and repair [3].

TUNEL assay on skeletal tissue sections to detect cell death / L. Mangiavini, E. Schipani (METHODS IN MOLECULAR BIOLOGY). - In: Skeletal Development and Repair : Methods and Protocols / [a cura di] M.J. Hilton. - [s.l] : Springer, 2014. - ISBN 9781627039888. - pp. 245-248 [10.1007/978-1-62703-989-5_18]

TUNEL assay on skeletal tissue sections to detect cell death

L. Mangiavini
Primo
;
2014

Abstract

At least two distinct modalities of cell death, apoptosis and necrosis, can be distinguished based on differences in morphological, biochemical, and molecular changes of dying cells [1, 2]. Cell death is involved both in physiological and pathological conditions of the skeleton: for example, apoptosis is a crucial event during limb development. Therefore, detection of cell death by using a simple stain is a powerful tool to study molecular and cellular mechanisms of skeletal development and repair [3].
Apoptosis; Necrosis; DNA; Terminal deoxynucleotidyltransferase; UTP; Immunofluorescence
Settore MED/33 - Malattie Apparato Locomotore
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/650673
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