To investigate skeletal development, pathophysiological mechanisms of cartilage and bone disease, and eventually assess innovative treatments, the mouse is a very important resource. During embryonic development, mesenchymal condensations are formed, and cells within these mesenchymal condensations either directly differentiate into osteoblasts and give origin to intramembranous bone, or differentiate into chondrocytes and form a cartilaginous anlage. The cartilaginous anlage or fetal growth plate is then replaced with bone. This process is also called endochondral bone development, and it is responsible for the generation of most of our skeleton. Here we discuss in detail the most common in vivo and in vitro techniques our laboratory is currently using for the analysis of the mouse fetal growth plate during development.
Analysis of Mouse Growth Plate Development / L. Mangiavini, C. Merceron, E. Schipani. - In: CURRENT PROTOCOLS IN MOUSE BIOLOGY. - ISSN 2161-2617. - 6:1(2016 Mar 01), pp. 67-130.
|Titolo:||Analysis of Mouse Growth Plate Development|
MANGIAVINI, LAURA (Primo)
|Settore Scientifico Disciplinare:||Settore MED/33 - Malattie Apparato Locomotore|
|Data di pubblicazione:||1-mar-2016|
|Data ahead of print / Data di stampa:||giu-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/9780470942390.mo150094|
|Appare nelle tipologie:||01 - Articolo su periodico|