The customary dilution of boar semen for subsequent artificial insemination (AI) procedures damages the cell membrane of spermatozoa, resulting in a loss of enzymes and other cytoplasmic contents and acrosomal reactions. We encapsulated non-diluted boar semen in barium alginate membranes to optimize AI procedures and to improve the functional integrity of spermatozoal membranes during storage. The percentage of non-reacted acrosomes (NRA) and measurements of enzyme leakage (cytochrome c oxidase (COX), lactate dehydrogenase (LDH), and glucose-6-phosphate dehydrogenase (G6PDH)) were used as indices of the functional status of diluted, unencapsulated and encapsulated spermatozoa, stored for 72 h at 18°C. Enzymatic activity was assessed in situ by microdensitometry, and non-reacted acrosomes were microscopically determined by staining. The percentage of acrosome integrity and the intracellular enzymatic activities during storage were different for unencapsulated and encapsulated semen. Semen dilution caused a rapid decline in enzymatic activities and concomitant acrosomal reactions. Encapsulated spermatozoa had significantly higher acrosome integrity (77% versus 55%; P < 0.01 after 72 h) and an overall higher in situ enzymatic activity. For cytochrome c oxidase and lactate dehydrogenase the greatest differences between encapsulated and unencapsulated spermatozoa were present after 72 h whereas for glucose-6-phosphate dehydrogenase significant differences were found within 24 h of storage. The encapsulation process maintains a better preservation environment for boar spermatozoa and could be a promising, innovative technique to improve storage of these cells.
Boar spermatozoa encapsulated in barium alginate membranes : a microdensitometric evaluation of some enzymatic activities during storage at 18°C / M. Faustini, M.L. Torre, S. Stacchezzini, R. Norberti, A. Lange Consiglio, F. Porcelli, U. Conte, E. Munari, V. Russo, D. Vigo. - In: THERIOGENOLOGY. - ISSN 0093-691X. - 61:1(2004 Jan 01), pp. 173-184.
|Titolo:||Boar spermatozoa encapsulated in barium alginate membranes : a microdensitometric evaluation of some enzymatic activities during storage at 18°C|
FAUSTINI, MASSIMO (Primo)
RUSSO, VINCENZO (Penultimo)
VIGO, DANIELE (Ultimo)
|Parole Chiave:||Swine ; Spermatozoa ; Encapsulation ; Enzyme leakage ; Cell membrane|
|Settore Scientifico Disciplinare:||Settore VET/02 - Fisiologia Veterinaria|
|Data di pubblicazione:||1-gen-2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0093-691X(03)00203-6|
|Appare nelle tipologie:||01 - Articolo su periodico|