A collection of 1373 unique flanking sequence tags (FSTs), generated from Ac/Ds and Ac transposon lines for genetics studies, were produced in japonica and indica rice, respectively. The Ds and Ac FSTs together with the original T-DNAs were assigned a position in the rice genome sequence represented as assembled pseudomolecules, and found to be distributed evenly over the entire rice genome with a distinct bias for predicted gene-rich regions. The bias of the Ds and Ac transposon inserts for genes was exemplified by the presence of 59% of the inserts in genes annotated on the rice chromosomes and 41% present in genes transcribed as disclosed by their homology to cDNA clones. In a screen for inserts in a set of 75 well annotated transcription factors, including homeobox-containing genes, we found six Ac/Ds inserts. This high frequency of Ds and Ac inserts in genes suggests that saturated knockout mutagenesis in rice using this strategy will be efficient and possible with a lower number of inserts than expected. These FSTs and the corresponding plant lines are publicly available through OrygenesDB database and from the EU consortium members.
|Titolo:||EU-OSTID : a collection of transposon insertional mutants for functional genomics in rice|
|Parole Chiave:||functional genomics ; mutants ; reverse genetics ; rice ; transposons|
|Settore Scientifico Disciplinare:||Settore BIO/01 - Botanica Generale|
|Data di pubblicazione:||set-2005|
|Digital Object Identifier (DOI):||10.1007/s11103-005-8532-6|
|Appare nelle tipologie:||01 - Articolo su periodico|