A protein named QP47 has been purified from quiescent nuclei of root meristems of Pisum sativum, and used to prepare a polyclonal antibody. Immunolocalization of this protein with fluorescent probes revealed a nuclear distribution of thread-like structures. However, the relationship between the distribution of QP47 immunofluorescence and the structural organization of the chromatin required further investigation. The decrease in content of this protein in the nuclei of embryo cells seems to be correlated with the transition from quiescence to proliferation. QP47 degradation seems to depend upon an increase in the state of its phosphorylation. This protein is not present in normally proliferating cells, or in cells whose cell proliferation has been arrested by starvation or differentiation. It is hypothesized that QP47 may be required specifically during the quiescent period for specific structural organization of the chromatin.
|Titolo:||Nuclear proteins and the onset of cell proliferation in root meristems of Pisum sativum: QP47 a novel acidic protein|
ONELLI, ELISABETTA (Ultimo)
|Parole Chiave:||Nuclear proteins ; cell proliferation ; quiescence ; germination ; Pisum sativum|
|Data di pubblicazione:||1993|
|Digital Object Identifier (DOI):||10.1017/S0960258500001549|
|Appare nelle tipologie:||01 - Articolo su periodico|