OBJECTIVES: We evaluated the possibility that a pattern of abnormal microRNA (miRNA) expression could be fuelling the mechanisms causing HIV-associated lipodystrophy (HAL).METHODS: In this case-control study, samples of subcutaneous adipose tissue from eight consecutive HIV-infected patients on combination antiretroviral therapy with HAL (cases) were compared with those of eight HIV-negative subjects (controls). Human miRNA microarrays were used to probe the transcriptomes of the samples. Analysis of differentially expressed miRNAs was performed using DataAssist v2.0 software, applying a paired Student's t-test.RESULTS: Data showed that 21 miRNAs out of 754 were overexpressed in the patient group. Ten of these (i.e. miR-186, miR-199a-3p, miR-214, miR-374a, miR-487b, miR-532-5p, miR-628-5p, miR-874, miR-125-b-1* and miR-374b*) were up-regulated to a significant degree (fold change >2.5; P < 0.01). Eleven other miRNAs (i.e. miR-let-7d, miR-24, miR-30c, miR-125a-3p, miR-149, miR-191, miR-196-b, miR-218, miR-342-3p, miR-452 and miR-454*) were 2- to 2.5-fold more expressed in HIV+ samples than in controls. Levels of mRNA for lipin 1, the target of miR-218, were significantly lower in subcutaneous adipose tissue from HIV patients.CONCLUSIONS: In adipocytes of HIV-infected patients, the up-regulation of specific miRNAs could lead to an increased 'activation' that might contribute to the pathogenesis of HAL by increasing cell turnover and/or promotion of apoptosis.
Changes in subcutaneous adipose tissue microRNA expression in HIV-infected patients / N. Squillace, E. Bresciani, A. Torsello, A. Bandera, F. Sabbatini, C. Giovannetti, G. Giunta, L. Rovati, M. Del Bene, V. Locatelli, A. Gori. - In: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. - ISSN 1460-2091. - 69:11(2014), pp. 3067-3075.
|Titolo:||Changes in subcutaneous adipose tissue microRNA expression in HIV-infected patients|
|Parole Chiave:||adipose tissue abnormalities; HIV-associated lipodystrophy; miRNAs; Adult; Aged; Antiretroviral Therapy; Highly Active; Case-Control Studies; Gene Expression Regulation; HIV Infections; Humans; Male; MicroRNAs; Middle Aged; Subcutaneous Fat; Medicine (all)|
|Settore Scientifico Disciplinare:||Settore MED/17 - Malattie Infettive|
|Data di pubblicazione:||2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/jac/dku264|
|Appare nelle tipologie:||01 - Articolo su periodico|