BackgroundThe dipstick is a first-line and inexpensive test that can exclude the presence of proteinuria in dogs. However, no information is available about the analytical variability of canine urine dipstick analysis. ObjectivesThe aim of this study was to assess the analytical variability in 2 dipsticks and the inter-operator variability in dipstick interpretation. MethodsCanine urine supernatants (n = 174) were analyzed with 2 commercially available dipsticks. Two observers evaluated each result blinded to the other observer and to the results of the other dipstick. Intra- and inter-assay variability was assessed in 5 samples (corresponding to the 5 different semi-quantitative results) tested 10 consecutive times over 5 consecutive days. The agreement between observers and between dipsticks was evaluated with Cohen's k test. ResultsIntra-assay repeatability was good (3/10 errors), whereas inter-assay variability was higher (from 1/5 to 4/5 discordant results). The concordance between the operators (k = 0.68 and 0.79 for the 2 dipsticks) and that of the dipsticks (k = 0.66 and 0.74 for the 2 operators) was good. However, 1 observer and 1 dipstick overestimated the results compared with the second observer or dipstick. In any case, discordant results accounted for a single unit of the semi-quantitative scale. ConclusionsAs for any other method, analytic variability may affect the semi-quantitation of urinary proteins when using the dipstick method. Subjective interpretation of the pad and, to a lesser extent, intrinsic staining properties of the pads could affect the results. Further studies are warranted to evaluate the effect of this variability on clinical decisions.

Evaluation of the analytical variability of dipstick protein pads in canine urine / M. Giraldi, S. Paltrinieri, A. Zatelli. - In: VETERINARY CLINICAL PATHOLOGY. - ISSN 0275-6382. - 47:2(2018 Jun), pp. 246-251. [10.1111/vcp.12594]

Evaluation of the analytical variability of dipstick protein pads in canine urine

M. Giraldi;S. Paltrinieri;A. Zatelli
2018-06

Abstract

BackgroundThe dipstick is a first-line and inexpensive test that can exclude the presence of proteinuria in dogs. However, no information is available about the analytical variability of canine urine dipstick analysis. ObjectivesThe aim of this study was to assess the analytical variability in 2 dipsticks and the inter-operator variability in dipstick interpretation. MethodsCanine urine supernatants (n = 174) were analyzed with 2 commercially available dipsticks. Two observers evaluated each result blinded to the other observer and to the results of the other dipstick. Intra- and inter-assay variability was assessed in 5 samples (corresponding to the 5 different semi-quantitative results) tested 10 consecutive times over 5 consecutive days. The agreement between observers and between dipsticks was evaluated with Cohen's k test. ResultsIntra-assay repeatability was good (3/10 errors), whereas inter-assay variability was higher (from 1/5 to 4/5 discordant results). The concordance between the operators (k = 0.68 and 0.79 for the 2 dipsticks) and that of the dipsticks (k = 0.66 and 0.74 for the 2 operators) was good. However, 1 observer and 1 dipstick overestimated the results compared with the second observer or dipstick. In any case, discordant results accounted for a single unit of the semi-quantitative scale. ConclusionsAs for any other method, analytic variability may affect the semi-quantitation of urinary proteins when using the dipstick method. Subjective interpretation of the pad and, to a lesser extent, intrinsic staining properties of the pads could affect the results. Further studies are warranted to evaluate the effect of this variability on clinical decisions.
dogs; proteinuria; reagent strips; urinalysis
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Settore VET/08 - Clinica Medica Veterinaria
VETERINARY CLINICAL PATHOLOGY
Article (author)
File in questo prodotto:
File Dimensione Formato  
s1-ln2570047195844769-1939656818Hwf-91746988IdV-54703210125700471PDF_HI0001.pdf

accesso aperto

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 277.34 kB
Formato Adobe PDF
277.34 kB Adobe PDF Visualizza/Apri
Giraldi_et_al-2018-Veterinary_Clinical_Pathology.pdf

non disponibili

Tipologia: Publisher's version/PDF
Dimensione 420.35 kB
Formato Adobe PDF
420.35 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/641429
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 2
social impact