Phenoxyherbicides induce peroxisome proliferation, as do other structurally related or unrelated chemicals which were found to be nongenotoxic carcinogens in rodents. The widespread use of 2,4-dichlorophenoxyacetic acid (2,4-D) raised concerns over the potential to produce cancer, yet results from epidemiological and experimental studies do not provide definitive evidence that 2,4-D is carcinogenic. The objective of the present study was to verify whether 2,4-D might interfere with cell growth similar to other peroxisome proliferators using cultured avian hepatocytes. Primary hepatocyte cultures from 18-day-old chick embryos were exposed to 2,4-D for up to 72 h followed by an assessment of replicative DNA synthesis and apoptosis. Further, peroxisome proliferation was evaluated. Results showed that 2,4-D stimulated DNA synthesis and suppressed spontaneous apoptosis, while the effects on peroxisome proliferation were less evident.
|Titolo:||S-phase, apoptosis and peroxisome proliferation in avian hepatocyte cultures following exposure to the phenoxy herbicide 2,4-D|
ARIAS, ELIO (Primo)
|Parole Chiave:||Apoptosis; Chick embryo; Chlorophenoxy herbicides; Cultured avian hepatocytes; DNA synthesis; Peroxisome proliferators|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||2009|
|Appare nelle tipologie:||01 - Articolo su periodico|