Objective. To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB). Methods. Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay. Results. Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin. Conclusion. These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.
Concentration of autoantibodies to native 60‐kd ro/ss‐a and denatured 52‐kd ro/ss‐a in eluates from the heart of a child who died with congenital complete heart block / M. Reichlin, A. Brucato, M.B. Frank, P.J. Maddison, V.R. Mccubbin, M. Wolfson-Reichlin, L.A. Lee. - In: ARTHRITIS AND RHEUMATISM. - ISSN 0004-3591. - 37:11(1994), pp. 1698-1703.
Concentration of autoantibodies to native 60‐kd ro/ss‐a and denatured 52‐kd ro/ss‐a in eluates from the heart of a child who died with congenital complete heart block
A. Brucato;
1994
Abstract
Objective. To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB). Methods. Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay. Results. Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin. Conclusion. These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.File | Dimensione | Formato | |
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