Objective. To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB). Methods. Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay. Results. Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin. Conclusion. These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.

Concentration of autoantibodies to native 60‐kd ro/ss‐a and denatured 52‐kd ro/ss‐a in eluates from the heart of a child who died with congenital complete heart block / M. Reichlin, A. Brucato, M.B. Frank, P.J. Maddison, V.R. Mccubbin, M. Wolfson-Reichlin, L.A. Lee. - In: ARTHRITIS AND RHEUMATISM. - ISSN 0004-3591. - 37:11(1994), pp. 1698-1703.

Concentration of autoantibodies to native 60‐kd ro/ss‐a and denatured 52‐kd ro/ss‐a in eluates from the heart of a child who died with congenital complete heart block

A. Brucato;
1994

Abstract

Objective. To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB). Methods. Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay. Results. Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin. Conclusion. These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.
Neonatal lupus-erythematosus; connective-tissue disease; RO SS-A; sjogrens syndrome; antibodies; antigen; immunoglobulin; deposition; mothers; risk
Settore MED/09 - Medicina Interna
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/639367
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