We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro. Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.

Inhibition of the SUMO pathway by Gam1 / M. Pozzebon, C.V. Segré, S. Chiocca - In: SUMO protocols / [a cura di] H.D. Ulrich. - New York : Humana Press, 2009. - ISBN 978-1-934115-80-0. - pp. 285-301 [10.1007/978-1-59745-566-4]

Inhibition of the SUMO pathway by Gam1

M. Pozzebon
Primo
;
C.V. Segré
Secondo
;
2009

Abstract

We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro. Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.
Degradation; Gam1; Inhibition; SAE1/SAE2; SUMO; SUMO E1; SUMO E2; Sumoylation; UBC9
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/63841
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