Localization of chemotherapy at the tumor site can improve therapeutic efficacy and reduce systemic toxicity. In previous studies we have shown that mesenchymal stromal cells (MSCs) isolated from bone marrow or adipose tissue can be loaded with the anti-cancer drug Paclitaxel (PTX) and kill cancer cells when localized nearby. We here investigated the capacity of human micro-fragmented adipose tissue (MFAT), used as a natural scaffold of MSCs, to deliver PTX with the idea to improve local drug concentration and to prolong the therapeutic activity. Surprisingly, we found that both fresh but also devitalized MFAT (DMFAT) (by freezing/thawing procedure) were able to deliver and release significant amount of PTX, killing several human cancer cell lines in vitro with a long lasting activity. In an orthotopic mice model of Neuroblastoma (NB) transplant, DMFAT loaded with PTX prevents or delays NB relapse when placed in the surgical area of tumor resection, without any collateral toxicity. We concluded that MFAT, but also DMFAT, may represent very innovative natural biomaterials able to localize and release anti-cancer molecules at the tumor site, helping to fight cancer in human.

Microfragmented human fat tissue is a natural scaffold for drug delivery : potential application in cancer chemotherapy / G. Alessandri, V. Coccè, F. Pastorino, R. Paroni, M. Dei Cas, F. Restelli, B. Pollo, L. Gatti, C. Tremolada, A. Berenzi, E. Parati, A.T. Brini, G. Bondiolotti, M. Ponzoni, A. Pessina. - In: JOURNAL OF CONTROLLED RELEASE. - ISSN 0168-3659. - 302(2019 May), pp. 2-18. [10.1016/j.jconrel.2019.03.016]

Microfragmented human fat tissue is a natural scaffold for drug delivery : potential application in cancer chemotherapy

V. Coccè
Secondo
Investigation
;
R. Paroni
Writing – Review & Editing
;
M. Dei Cas
Investigation
;
L. Gatti
Investigation
;
C. Tremolada
Investigation
;
E. Parati
Investigation
;
A.T. Brini
Supervision
;
G. Bondiolotti;A. Pessina
Ultimo
Project Administration
2019

Abstract

Localization of chemotherapy at the tumor site can improve therapeutic efficacy and reduce systemic toxicity. In previous studies we have shown that mesenchymal stromal cells (MSCs) isolated from bone marrow or adipose tissue can be loaded with the anti-cancer drug Paclitaxel (PTX) and kill cancer cells when localized nearby. We here investigated the capacity of human micro-fragmented adipose tissue (MFAT), used as a natural scaffold of MSCs, to deliver PTX with the idea to improve local drug concentration and to prolong the therapeutic activity. Surprisingly, we found that both fresh but also devitalized MFAT (DMFAT) (by freezing/thawing procedure) were able to deliver and release significant amount of PTX, killing several human cancer cell lines in vitro with a long lasting activity. In an orthotopic mice model of Neuroblastoma (NB) transplant, DMFAT loaded with PTX prevents or delays NB relapse when placed in the surgical area of tumor resection, without any collateral toxicity. We concluded that MFAT, but also DMFAT, may represent very innovative natural biomaterials able to localize and release anti-cancer molecules at the tumor site, helping to fight cancer in human.
Anti-cancer chemotherapy; Biomaterials; Drug delivery; Micro-fragmented adipose tissue; Natural scaffold; 3003
Settore MED/07 - Microbiologia e Microbiologia Clinica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore AGR/19 - Zootecnica Speciale
Settore BIO/14 - Farmacologia
Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree
mag-2019
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/637904
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