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AIR nasce in UNIMI nel 2006 con lo scopo di raccogliere, documentare e conservare le informazioni sulla produzione scientifica dell'Università degli Studi di Milano e costituisce di fatto l'Anagrafe della ricerca dell'Ateneo.
EnglishProstate cancer cells release atypically large extracellular vesicles (EVs), termed large oncosomes, which may play a role in the tumor microenvironment by transporting bioactive molecules across tissue spaces and through the blood stream. In this study, we applied a novel method for selective isolation of large on cosomes applicable to human plateletpoor plasma, where the presence of caveolin-l-positive large oncosomes identified patients with metastatic disease. This procedure was also used to validate results of a miRNA array performed on heterogeneous populations of EVs isolated from tumorigenic RWPE-2 prostate cells and from isogenic non-tumorigenic RWPE-1 cells. The results showed that distinct classes of miRNAs are expressed at higher levels in EVs derived from the tumorigenic cells in comparison to their non-tumorigenic counterpart. Large oncosomes enhanced migration of cancer-associated fibroblasts (CAFs), an effect that was increased by miR-1227, a miRNA abundant in large oncosomes produced by RWPE-2 cells. Our findings suggest that large oncosomes in the circulation report metastatic disease in patients with prostate cancer, and that this class of EV harbors functional molecules that may play a role in conditioning the tumor microenvironment.
Large oncosomes mediate intercellular transfer of functional microRNA / M. Morello, V. Minciacchi, P. de Candia, J. Yang, E. Posadas, H. Kim, D. Griffiths, N. Bhowmick, L. Chung, P. Gandellini, M. Freeman, F. Demichelis, D. Di Vizio. - In: CELL CYCLE. - ISSN 1538-4101. - 12:22(2013), pp. 3526-3536.
| Titolo: | Large oncosomes mediate intercellular transfer of functional microRNA | |
| Autori: | ||
| Parole Chiave: | amoeboid blebbing; microvesicle; filtration; large oncosome; exosome; extracellular vesicles; miRNA; prostate cancer | |
| Settore Scientifico Disciplinare: | Settore MED/04 - Patologia Generale | |
| Data di pubblicazione: | 2013 | |
| Rivista: | ||
| Tipologia: | Article (author) | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.4161/cc.26539 | |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Large oncosomes mediate intercellular transfer of functional microRNA.pdf | Publisher's version/PDF | Open Access Visualizza/Apri |
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