The nucleolytic degradation of the 5′-ending strand of a Double-Strand DNA break (DSB) is necessary to initiate homologous recombination to correctly repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.
A qPCR-based protocol to quantify DSB resection / M. Ferrari, S. Twayana, F. Marini, A. Pellicioli (METHODS IN MOLECULAR BIOLOGY). - In: Genome Instability : Methods and Protocols / [a cura di] M. Muzi-Falconi, G. W Brown. - [s.l] : Humana, 2018. - ISBN 9781493973057. - pp. 119-129
A qPCR-based protocol to quantify DSB resection
S. Twayana;F. Marini;A. Pellicioli
Ultimo
2018
Abstract
The nucleolytic degradation of the 5′-ending strand of a Double-Strand DNA break (DSB) is necessary to initiate homologous recombination to correctly repair the break. This process is called DNA end resection and it is finely regulated to prevent genome rearrangements. Here, we describe a protocol to quantify DSB resection rate by qPCR, which could be applied to every organisms whenever the break site and its flanking region sequences are known.
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