Circulating immature surfactant protein B (proSP-B) forms emerged as the most reliable lung-specific circulating marker for alveolar-capillary membrane (ACM) dysfunction and for the overall clinical status of heart failure (HF). Notably, in terms of HF hospitalization, immature SP-B overwhelms the prognostic role of other most frequently used clinical parameters such as those related to lung dysfunction. The strong prognostic value of circulating proSP-B in HF suggests more widespread and possible systemic effects. Thus, we assessed the plasma distribution of proSP-B evaluating whether it exists in a lipoprotein-bound form and its impact on lipoprotein structure and function. ProSP-B forms were detectable in high-density lipoprotein (HDL) only. To assess the impact of proSP-B on HDL, HDL from healthy subjects were enriched with proSP-B produced by a stably transfected CHO cell line that specifically expresses and releases the human proSP-B. After enrichment, HDL size and lipoprotein electrophoretic mobility, and protein composition did not show apparent differences. HDL antioxidant capacity (HOI), assessed as their ability to inhibit air-induced LDL oxidation, was impaired after proSP-B enrichment. HOI was also higher in HF patients with respect to age-matched control healthy subjects (p = 0.013). Circulating proSP-B, besides its potential role as a specific marker for ACM dysfunction in HF patients with diagnostic and prognostic value, binds to human HDL impairing their antioxidant capacity. These findings shed light on proSP-B as a molecule that contributes to the reduction of the defense against oxidative stress, a key mediator in the pathogenesis of HF.
Immature surfactant protein-B impairs the antioxidant capacity of HDL / C. Banfi, M. Brioschi, M.K. Karjalainen, J.M. Huusko, E. Gianazza, P. Agostoni. - In: INTERNATIONAL JOURNAL OF CARDIOLOGY. - ISSN 0167-5273. - 285(2019 Jun 15), pp. 53-58.
|Titolo:||Immature surfactant protein-B impairs the antioxidant capacity of HDL|
BANFI, CRISTINA (Primo) (Corresponding)
BRIOSCHI, MAURA (Secondo)
AGOSTONI, PIERGIUSEPPE (Ultimo)
|Parole Chiave:||HDL; Heart failure; Oxidant index; Surfactant protein B; Cardiology and Cardiovascular Medicine|
|Settore Scientifico Disciplinare:||Settore MED/11 - Malattie dell'Apparato Cardiovascolare|
|Data di pubblicazione:||15-giu-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.ijcard.2019.02.057|
|Appare nelle tipologie:||01 - Articolo su periodico|