In this system the gene of interest is under transcriptional control of the Streptomyces Pip protein, the pristinamycin sensitive repressor. The pip gene of S. coelicolor has been cloned downstream of a constitutive mycobacterial promoter (the mutant pfurA102 of M. tb), and introduced in Mycobacterium smegmatis (see pMYS696 in Fig. 1). Furthermore, a Pip-ON system has been constructed, by cloning the S. pristinaespiralis ptr promoter upstream of a reporter gene (lacZ), in the above plasmid (see pMY718 in Fig. 1). These constructs have been introduced in M. smegmatis and beta-galactosidase activity measured: the activity expressed from pMY718 is very low (less than 10 Miller units) and inducible, in a dose dependent manner, by addition of pristinamycin.
|Titolo:||Assess the utility of the PIP-ON system in M. tb and overexpress or silence several mycobacterial genes|
FORTI, FRANCESCA (Primo)
GHISOTTI, DANIELA ERICA (Ultimo)
|Data di pubblicazione:||2007|
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
|Citazione:||Assess the utility of the PIP-ON system in M. tb and overexpress or silence several mycobacterial genes / F. Forti, D. Ghisotti. - [s.l] : null, 2007.|
|Appare nelle tipologie:||08 - Relazione interna o rapporto di ricerca|