sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.

Enzymatic Synthesis of Valuable Bioactive Compounds / I. Bassanini, D. Monti, J. Krejzová, V. Křen, S. Riva. ((Intervento presentato al convegno I chimici per le biotecnologie tenutosi a Milano nel 2019.

Enzymatic Synthesis of Valuable Bioactive Compounds

I. Bassanini
;
S. Riva
2019-02-22

Abstract

sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.
Settore CHIM/08 - Chimica Farmaceutica
Enzymatic Synthesis of Valuable Bioactive Compounds / I. Bassanini, D. Monti, J. Krejzová, V. Křen, S. Riva. ((Intervento presentato al convegno I chimici per le biotecnologie tenutosi a Milano nel 2019.
Conference Object
File in questo prodotto:
File Dimensione Formato  
Bassanini_2019_ChimiciBiotech.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 196.14 kB
Formato Adobe PDF
196.14 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/631031
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact