sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.

Enzymatic Synthesis of Valuable Bioactive Compounds / I. Bassanini, D. Monti, J. Krejzová, V. Křen, S. Riva. ((Intervento presentato al convegno I chimici per le biotecnologie tenutosi a Milano nel 2019.

Enzymatic Synthesis of Valuable Bioactive Compounds

I. Bassanini
;
S. Riva
2019

Abstract

sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.
22-feb-2019
Settore CHIM/08 - Chimica Farmaceutica
Enzymatic Synthesis of Valuable Bioactive Compounds / I. Bassanini, D. Monti, J. Krejzová, V. Křen, S. Riva. ((Intervento presentato al convegno I chimici per le biotecnologie tenutosi a Milano nel 2019.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/631031
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