sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.
Enzymatic Synthesis of Valuable Bioactive Compounds / I. Bassanini, D. Monti, J. Krejzová, V. Křen, S. Riva. ((Intervento presentato al convegno I chimici per le biotecnologie tenutosi a Milano nel 2019.
Enzymatic Synthesis of Valuable Bioactive Compounds
I. Bassanini
;S. Riva
2019
Abstract
sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols was developed (Scheme 1). The reaction scheme was based on a transrutinosylation catalyzed by a rutinosidase from A. niger using the cheap and commercially available flavonoid rutin as glycosyl donor, followed by a selective ‘trimming’ of the rutinoside unit, catalyzed by a rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. This process allowed the facile preparation of several natural bioactive glucosides, which could be isolated in up to 80% yield without the need of silica-gel chromatography.File | Dimensione | Formato | |
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