Endothelial Differentiation Factor (EDF)-1 is a calmodulin binding protein involved in the repression of endothelial cell differentiation, a crucial, late step in angiogenesis. Its expression is cell cycle regulated, although its transcriptional regulation is yet to be determined. To map the promoter region and to understand its regulation, we cloned and fused 2300 bp upstream of EDF-1 translational start site to a luciferase reporter gene. After transient transfection in HeLa cells, this fragment was shown to possess a promoter activity. Deletion constructs of the 5' flanking region of EDF-1 lead to the identification of the minimal promoter region which was highly homologous to the mouse sequence. No TATA box was detected, whereas three consensus sequences--two GC boxes and a CAAT box--were identified. EMSA supershift and chromatin immunoprecipitation demonstrated that these sequences were binding sites for Sp1/Sp3 and NFY, respectively. Deletion of Sp1/Sp3 and NF-Y consensus sequences resulted in the total loss of EDF-1 promoter activity. Our studies indicate that Sp1 and NFY binding is essential for EDF-1 promoter activity.

Characterization of the human EDF-1 minimal promoter : involvement of NFY and Sp1 in the regulation of basal transcription / F. Bolognese, M. Pitarque-Marti, V. Lo Cicero, R. Mantovani, J.A. Maier. - In: GENE. - ISSN 0378-1119. - 374:1-2(2006 Jun 07), pp. 87-95.

Characterization of the human EDF-1 minimal promoter : involvement of NFY and Sp1 in the regulation of basal transcription

BOLOGNESE, FABRIZIO;LO CICERO, VIVIANA GIOVANNA;R. Mantovani;J.A. Maier
2006-06-07

Abstract

Endothelial Differentiation Factor (EDF)-1 is a calmodulin binding protein involved in the repression of endothelial cell differentiation, a crucial, late step in angiogenesis. Its expression is cell cycle regulated, although its transcriptional regulation is yet to be determined. To map the promoter region and to understand its regulation, we cloned and fused 2300 bp upstream of EDF-1 translational start site to a luciferase reporter gene. After transient transfection in HeLa cells, this fragment was shown to possess a promoter activity. Deletion constructs of the 5' flanking region of EDF-1 lead to the identification of the minimal promoter region which was highly homologous to the mouse sequence. No TATA box was detected, whereas three consensus sequences--two GC boxes and a CAAT box--were identified. EMSA supershift and chromatin immunoprecipitation demonstrated that these sequences were binding sites for Sp1/Sp3 and NFY, respectively. Deletion of Sp1/Sp3 and NF-Y consensus sequences resulted in the total loss of EDF-1 promoter activity. Our studies indicate that Sp1 and NFY binding is essential for EDF-1 promoter activity.
angiogenesis ; endothelium ; promoter ; HIV-Tat
Settore MED/04 - Patologia Generale
Settore BIO/18 - Genetica
GENE
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/62811
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