Seeds derive from ovules upon fertilization and therefore the total number of ovules determines the final seed yield, a fundamental trait in crop plants. Among the factors that co-ordinate the process of ovule formation, the transcription factors CUP-SHAPED COTYLEDON 1 (CUC1) and CUC2 and the hormone cytokinin (CK) have a particularly prominent role. Indeed, the absence of both CUC1 and CUC2 causes a severe reduction in ovule number, a phenotype that can be rescued by CK treatment. In this study, we combined CK quantification with an integrative genome-wide target identification approach to select Arabidopsis genes regulated by CUCs that are also involved in CK metabolism. We focused our attention on the functional characterization of UDP-GLUCOSYL TRANSFERASE 85A3 (UGT85A3) and UGT73C1, which are up-regulated in the absence of CUC1 and CUC2 and encode enzymes able to catalyse CK inactivation by O-glucosylation. Our results demonstrate a role for these UGTs as a link between CUCs and CK homeostasis, and highlight the importance of CUCs and CKs in the determination of seed yield.
CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 regulate cytokinin homeostasis to determine ovule number in Arabidopsis / M. Cucinotta, S. MANRIQUE URPI, C. Cuesta, E. Benkova, O. Novak, L. Colombo. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - 69:21(2018 Oct), pp. 5169-5176.
|Titolo:||CUP-SHAPED COTYLEDON1 (CUC1) and CUC2 regulate cytokinin homeostasis to determine ovule number in Arabidopsis|
CUCINOTTA, MARA (Primo)
MANRIQUE URPI, SILVIA (Secondo)
COLOMBO, LUCIA (Ultimo) (Corresponding)
|Parole Chiave:||Arabidopsis; cytokinin; hormones; ovule development; pistil development; transcriptional regulation|
|Settore Scientifico Disciplinare:||Settore BIO/01 - Botanica Generale|
|Data di pubblicazione:||ott-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/jxb/ery281|
|Appare nelle tipologie:||01 - Articolo su periodico|