Objective The major challenge for contemporary dentistry is restoration of missing teeth; currently, dental implantation is the treatment of choice in this circumstance. In the present study, we assessed the interaction between implants and Dental Pulp Stem Cells (DPSCs) in vitro by means of 3D cell culture in order to better simulate physiological conditions. Methods Sorted CD34+DPSCs were seeded onto dental implants having either a rough surface (TriVent) or one coated with a ceramic layer mimicking native bone (TiUnite). We evaluated preservation of DPSC viability during osteogenic differentiation by an MTT assay and compared mineralized matrix deposition with SEM analysis and histological staining; temporal expression of osteogenic markers was evaluated by RT-PCR and ELISA. Results Both surfaces are equally biocompatible, preserve DPSC viability, stimulate osteogenic differentiation, and increase the production of VEGF. A slight difference was observed between the two surfaces concerning the speed of DPSC differentiation. Significance Our study of the two implant surfaces suggests that TriVent, with its roughness, is capable of promoting cell differentiation a bit earlier than the TiUnite surface, although the latter promotes greater cell proliferation.
Surface biocompatibility of differently textured titanium implants with mesenchymal stem cells / P. Naddeo, L. Laino, M. La Noce, A. Piattelli, A. De Rosa, G. Iezzi, G. Laino, F. Paino, G. Papaccio, V. Tirino. - In: DENTAL MATERIALS. - ISSN 0109-5641. - 31:3(2015), pp. 235-243.
|Titolo:||Surface biocompatibility of differently textured titanium implants with mesenchymal stem cells|
PAINO, FRANCESCA (Corresponding)
|Parole Chiave:||Biocompatibility; Cell proliferation; DPSCs; Osteodifferentiation; Surface texture; Titanium implants; Biocompatible Materials; Cell Adhesion; Cell Differentiation; Cell Proliferation; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Humans; In Vitro Techniques; Mesenchymal Stromal Cells; Microscopy, Electron, Scanning; Osteogenesis; Polymerase Chain Reaction; Surface Properties; Titanium; Dental Implants; Materials Science (all); Dentistry (all); Mechanics of Materials|
|Settore Scientifico Disciplinare:||Settore BIO/17 - Istologia|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.dental.2014.12.015|
|Appare nelle tipologie:||01 - Articolo su periodico|