Dental pulp stem cells (DPSCs) are multipotent stem cells derived from neural crest and mesenchyme and have the capacity to differentiate into multiple cell lineages. It has already been demonstrated that DPSCs differentiate into melanocyte-like cells but only when cultivated in a specific melanocyte differentiating medium. In this study we have shown, for the first time, that DPSCs are capable of spontaneously differentiating into mature melanocytes, which display molecular and ultrastructural features of full development, including the expression of melanocyte specific markers and the presence of melanosomes up to the terminal stage of maturation. We have also compared the differentiating features of DPSCs grown in different culture conditions, following the timing of differentiation at molecular and cytochemical levels and found that in all culture conditions full development of these cells was obtained, although at different times. The spontaneous differentiating potential of these cells strongly suggests their possible applications in regenerative medicine.

Ecto-mesenchymal stem cells from dental pulp are committed to differentiate into active melanocytes / F. Paino, G. Ricci, A. De Rosa, R. D'Aquino, L. Laino, G. Pirozzi, V. Tirino, G. Papaccio. - In: EUROPEAN CELLS & MATERIALS. - ISSN 1473-2262. - 20(2010), pp. 295-305.

Ecto-mesenchymal stem cells from dental pulp are committed to differentiate into active melanocytes

F. Paino;
2010

Abstract

Dental pulp stem cells (DPSCs) are multipotent stem cells derived from neural crest and mesenchyme and have the capacity to differentiate into multiple cell lineages. It has already been demonstrated that DPSCs differentiate into melanocyte-like cells but only when cultivated in a specific melanocyte differentiating medium. In this study we have shown, for the first time, that DPSCs are capable of spontaneously differentiating into mature melanocytes, which display molecular and ultrastructural features of full development, including the expression of melanocyte specific markers and the presence of melanosomes up to the terminal stage of maturation. We have also compared the differentiating features of DPSCs grown in different culture conditions, following the timing of differentiation at molecular and cytochemical levels and found that in all culture conditions full development of these cells was obtained, although at different times. The spontaneous differentiating potential of these cells strongly suggests their possible applications in regenerative medicine.
Dental pulp stem cells; Differentiation; L-Dopa; Melanocytes; Melanosomes; Bioengineering; Biochemistry; Biomaterials; Biomedical Engineering; Cell Biology
Settore BIO/17 - Istologia
Settore BIO/16 - Anatomia Umana
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/625571
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