Pole3 (DPB4/YBL1/CHRAC17) is one of the subunits of the DNA polymerase e. It contains a histone-like domain required for the hererodimerization with its Pole4 (DPB3) partner. In another interaction, Pole3 heterodimerizes with YCL1/CHRAC15 and associates with the ACF1/SNF2H remodelling complex. We find that the Pol3 gene is regulated in starved NIH3T3 fibroblasts upon induction with serum, with a peak at the entry in the S phase. We characterized the Pole3 promoter, which is linked bidirectionally to C90rf46, a gene of unknown function: it has no CCAAT nor TATA-boxes, and contains an E box and two potential E2F sites. Mutagenesis analysis points to a minimal promoter region as sufficient for activation; the E box and a neighbouring direct repeat are important for regulation. Cell-cycle regulation was reproduced in stable clones and an additional E2F site was found to be important. Chromatin immunoprecipitation analysis indicates that E2F1/4, as well as MYC, are associated with the Pole3 promoter in a phase-specific way. These data highlight coregulation of a histone-like gene with core histones upon DNA synthesis, and represent a first dissection of the interplay between two essential cell-cycle regulators on a bidirectional promoter.
The Pole3 bidirectional unit is regulated by MYC and E2Fs / F. Bolognese, C. Forni, G. Caretti, M. Frontini, M. Minuzzo, R. Mantovani. - In: GENE. - ISSN 0378-1119. - 366:1(2006 Jan 17), pp. 109-116.
|Titolo:||The Pole3 bidirectional unit is regulated by MYC and E2Fs|
BOLOGNESE, FABRIZIO (Primo)
FORNI, CLAUDIA (Secondo)
MINUZZO, MARIO SILVIO (Penultimo)
MANTOVANI, ROBERTO (Ultimo)
|Parole Chiave:||transcription ; promoter ; DNA polymerase|
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
|Data di pubblicazione:||17-gen-2006|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.gene.2005.07.046|
|Appare nelle tipologie:||01 - Articolo su periodico|