We previously found that human amniotic mesenchymal stem cells (hAMSCs) in coculture with CF immortalised airway epithelial cells (CFBE41o-line, CFBE) on Transwell® filters acquired an epithelial phenotype and led to the expression of a mature and functional CFTR protein. In order to explore the role of gap junction-(GJ-) mediated intercellular communication (GJIC) in this rescue, cocultures (hAMSC: CFBE, 1: 5 ratio) were studied for the formation of GJIC, before and after silencing connexin 43 (Cx43), a major component of GJs. Functional GJs in cocultures were inhibited when the expression of the Cx43 protein was downregulated. Transfection of cocultures with siRNA against Cx43 resulted in the absence of specific CFTR signal on the apical membrane and reduction in the mature form of CFTR (band C), and in parallel, the CFTR-dependent chloride channel activity was significantly decreased. Cx43 downregulation determined also a decrease in transepithelial resistance and an increase in paracellular permeability as compared with control cocultures, implying that GJIC may regulate CFTR expression and function that in turn modulate airway epithelium tightness. These results indicate that GJIC is involved in the correction of CFTR chloride channel activity upon the acquisition of an epithelial phenotype by hAMSCs in coculture with CF cells.
Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o-Cells / A. Carbone, R. Zefferino, E. Beccia, V. Casavola, S. Castellani, S. Di Gioia, V. Giannone, M. Seia, A. Angiolillo, C. Colombo, M. Favia, M. Conese. - In: STEM CELLS INTERNATIONAL. - ISSN 1687-9678. - 2018(2018).
|Titolo:||Gap Junctions Are Involved in the Rescue of CFTR-Dependent Chloride Efflux by Amniotic Mesenchymal Stem Cells in Coculture with Cystic Fibrosis CFBE41o-Cells|
|Parole Chiave:||Molecular Biology; Cell Biology|
|Settore Scientifico Disciplinare:||Settore MED/38 - Pediatria Generale e Specialistica|
Settore BIO/11 - Biologia Molecolare
|Data di pubblicazione:||2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1155/2018/1203717|
|Appare nelle tipologie:||01 - Articolo su periodico|