Many signal perception mechanisms are connected to Ca2+-based second messenger signaling to modulate specific cellular responses. The well-characterized plant hormone auxin elicits a very rapid Ca2+ signal. However, the cellular targets of auxin-induced Ca2+ are largely unknown. Here, we screened a biologically annotated chemical library for inhibitors of auxin-induced Ca2+ entry in plant cell suspensions to better understand the molecular mechanism of auxin-induced Ca2+ and to explore the physiological relevance of Ca2+ in auxin signal transduction. Using this approach, we defined a set of diverse, small molecules that interfere with auxin-induced Ca2+ entry. Based on annotated biological activities of the hit molecules, we found that auxin-induced Ca2+ signaling is, among others, highly sensitive to disruption of membrane proton gradients and the mammalian Ca2+ channel inhibitor bepridil. Whereas protonophores nonselectively inhibited auxin-induced and osmotic-stress-induced Ca2+ signals, bepridil specifically inhibited auxin-induced Ca2+. We found evidence that bepridil severely alters vacuolar morphology and antagonized auxin-induced vacuolar remodeling. Further exploration of this plant-tailored collection of inhibitors will lead to a better understanding of auxin-induced Ca2+ entry and its relevance for auxin responses.
Identification of novel inhibitors of auxin-induced Ca2+ signaling via a plant-based chemical screen / K. De Vriese, E. Himschoot, K. Dunser, L. Nguyen, A. Drozdzecki, A. Costa, M.K. Nowack, J. Kleine-Vehn, D. Audenaert, T. Beeckman, S. Vanneste. - In: PLANT PHYSIOLOGY. - ISSN 0032-0889. - 180:1(2019 May), pp. 480-496.
|Titolo:||Identification of novel inhibitors of auxin-induced Ca2+ signaling via a plant-based chemical screen|
COSTA, ALEX [Writing – Review & Editing]
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
|Data di pubblicazione:||mag-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1104/pp.18.01393|
|Appare nelle tipologie:||01 - Articolo su periodico|