Mucositis is a frequent problem after irradiation of the oral mucosa. To study the early effects of irradiation on the desmosomal adhesion complex, explants of keratinized oral mucosa were exposed to a single dose of 2 Gy gamma irradiation. Biopsies were obtained from the upper dental arch of nine young healthy non-smoking women undergoing minor oral surgery. The biopsies were incubated in a Transwell culture system and, after irradiation, fixed in formalin 24 h later. Morphometric measurements of epithelial thickness revealed that it was unaffected by exposure to ionizing rays. Immunofluorescence analysis of desmosomal cadherin expression (desmoglein 1/desmoglein 3) demonstrated that the distribution of desmoglein 1 was not affected, whereas the expression of desmoglein 3 decreased in the suprabasal layers of irradiated samples. It is suggested that this has consequences for the mechanical integrity of the mucosa and promotes the development of radiation mucositis.

An in vitro model of human oral explants to study early effects of radiation mucositis / E. Donetti, M. Bedoni, P. Capone, A. Gualerzi, G. Tartaglia, C. Sforza. - In: EUROPEAN JOURNAL OF ORAL SCIENCES. - ISSN 0909-8836. - 117:2(2009 Apr), pp. 169-174. [10.1111/j.1600-0722.2009.00614.x]

An in vitro model of human oral explants to study early effects of radiation mucositis

E. Donetti
Primo
;
M. Bedoni
Secondo
;
G. Tartaglia
Penultimo
;
C. Sforza
Ultimo
2009

Abstract

Mucositis is a frequent problem after irradiation of the oral mucosa. To study the early effects of irradiation on the desmosomal adhesion complex, explants of keratinized oral mucosa were exposed to a single dose of 2 Gy gamma irradiation. Biopsies were obtained from the upper dental arch of nine young healthy non-smoking women undergoing minor oral surgery. The biopsies were incubated in a Transwell culture system and, after irradiation, fixed in formalin 24 h later. Morphometric measurements of epithelial thickness revealed that it was unaffected by exposure to ionizing rays. Immunofluorescence analysis of desmosomal cadherin expression (desmoglein 1/desmoglein 3) demonstrated that the distribution of desmoglein 1 was not affected, whereas the expression of desmoglein 3 decreased in the suprabasal layers of irradiated samples. It is suggested that this has consequences for the mechanical integrity of the mucosa and promotes the development of radiation mucositis.
5-bromo-2′-deoxyuridine; Desmoglein 1; Desmoglein 3; Human oral keratinocytes; Ionizing rays
Settore BIO/16 - Anatomia Umana
apr-2009
16-mar-2009
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/62179
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