Synthetic oligonucleotides corresponding to specific V3 loop portions of two HIV-1 isolates, SC and WMJ2, were expressed in the flagella of a Salmonella live-vaccine strain. Expression of the inserted epitopes in flagellin and their exposure at the surface of flagellar filaments were shown by immunoblotting and immunogold labeling with anti-flagellin (Salmonella d) and anti-HIV-1(IIIB) V3 loop peptide sera. Live recombinant Salmonella strains expressing either one of the two V3 loop inserts were administered intraperitoneally to BALB:c mice. All these animals developed antibodies specific for the heterologous glycoprotein 120 (gp120) of HIV-1 MN strain, as detected by enzyme -linked immunosorbent assays (ELISA); two of the sera had neutralizing activity against the heterologous HIV-1 MN strain. Moreover, oral administration of the live Salmonella recombinant strains to mice evoked specific IgA directed against gp120.
|Titolo:||Expression and immunogenicity of V3 loop epitopes of HIV-1, isolates SC and WMJ2, inserted in Salmonella flagellin|
DE GIULI MORGHEN, CARLO (Penultimo)
|Parole Chiave:||AroA- Salmonella; Flagellin; Gp120; V3 loop|
|Data di pubblicazione:||8-ago-1997|
|Digital Object Identifier (DOI):||10.1016/S0168-1656(97)00117-X|
|Appare nelle tipologie:||01 - Articolo su periodico|