The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays.

The nucleoid as a scaffold for the assembly of bacterial signaling complexes / A. Moine, L. Espinosa, E. Martineau, M. Yaikhomba, P. Jazleena, D. Byrne, E. Biondi, E. Notomista, M. Brilli, V. Molle, P. Gayathri, T. Mignot, E. Mauriello. - In: PLOS GENETICS. - ISSN 1553-7404. - 13:11(2017 Nov), pp. e1007103.1-e1007103.20. [10.1371/journal.pgen.1007103]

The nucleoid as a scaffold for the assembly of bacterial signaling complexes

M. Brilli;
2017

Abstract

The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. In this work, we show that FrzCD directly binds to the nucleoid with its N-terminal positively charged tail and recruits active signaling complexes at this location. The FrzCD binding to the nucleoid occur in a DNA-sequence independent manner and leads to the formation of multiple distributed clusters that explore constrained areas. This organization might be required for cooperative interactions between clustered receptors as observed in membrane-bound chemosensory arrays.
Settore BIO/19 - Microbiologia Generale
Settore BIO/18 - Genetica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/620643
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