A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.

The rise and rise of nicotiana benthamiana : a plant for all reasons / J. Bally, H. Jung, C. Mortimer, F. Naim, J.G. Philips, R. Hellens, A. Bombarely, M.M. Goodin, P.M. Waterhouse. - In: ANNUAL REVIEW OF PHYTOPATHOLOGY. - ISSN 0066-4286. - 56:1(2018), pp. 405-426. [10.1146/annurev-phyto-080417-050141]

The rise and rise of nicotiana benthamiana : a plant for all reasons

A. Bombarely;
2018

Abstract

A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.
English
agroinfiltration; biofactory; cellular localization; Nicotiana benthamiana; polyploid genome; VIGS; virus-induced gene silencing; Plant Science
Settore BIO/01 - Botanica Generale
Review essay
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
2018
Annual Reviews
56
1
405
426
22
Pubblicato
Periodico con rilevanza internazionale
scopus
NON aderisco
info:eu-repo/semantics/article
The rise and rise of nicotiana benthamiana : a plant for all reasons / J. Bally, H. Jung, C. Mortimer, F. Naim, J.G. Philips, R. Hellens, A. Bombarely, M.M. Goodin, P.M. Waterhouse. - In: ANNUAL REVIEW OF PHYTOPATHOLOGY. - ISSN 0066-4286. - 56:1(2018), pp. 405-426. [10.1146/annurev-phyto-080417-050141]
none
Prodotti della ricerca::01 - Articolo su periodico
9
262
Article (author)
si
J. Bally, H. Jung, C. Mortimer, F. Naim, J.G. Philips, R. Hellens, A. Bombarely, M.M. Goodin, P.M. Waterhouse
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/619711
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