A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.

The rise and rise of nicotiana benthamiana : a plant for all reasons / J. Bally, H. Jung, C. Mortimer, F. Naim, J.G. Philips, R. Hellens, A. Bombarely, M.M. Goodin, P.M. Waterhouse. - In: ANNUAL REVIEW OF PHYTOPATHOLOGY. - ISSN 0066-4286. - 56:1(2018), pp. 405-426. [10.1146/annurev-phyto-080417-050141]

The rise and rise of nicotiana benthamiana : a plant for all reasons

A. Bombarely;
2018

Abstract

A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.
agroinfiltration; biofactory; cellular localization; Nicotiana benthamiana; polyploid genome; VIGS; virus-induced gene silencing; Plant Science
Settore BIO/01 - Botanica Generale
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/619711
Citazioni
  • ???jsp.display-item.citation.pmc??? 49
  • Scopus 87
  • ???jsp.display-item.citation.isi??? 83
social impact