White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding protein that regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because of the upregulation of genes promoting lipolytic activity. Expression of microRNA 145 (miR-145) is decreased because of impaired primary miR-145 processing in Ksrp-/- eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through posttranscriptional regulation of miR-145 expression.
KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue / Y. Lin, C. Chou, M. Giovarelli, P. Briata, R. Gherzi, C. Chen. - In: MOLECULAR AND CELLULAR BIOLOGY. - ISSN 0270-7306. - 34:12(2014), pp. 2339-2349. [10.1128/MCB.00042-14]
KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue
M. Giovarelli;
2014
Abstract
White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding protein that regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because of the upregulation of genes promoting lipolytic activity. Expression of microRNA 145 (miR-145) is decreased because of impaired primary miR-145 processing in Ksrp-/- eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through posttranscriptional regulation of miR-145 expression.File | Dimensione | Formato | |
---|---|---|---|
2339.full.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
2.81 MB
Formato
Adobe PDF
|
2.81 MB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.