White adipose tissue (WAT) releases fatty acids from stored triacylglycerol for an energy source. Here, we report that targeted deletion of KH-type splicing regulatory protein (KSRP), an RNA-binding protein that regulates gene expression at multiple levels, enhances lipolysis in epididymal WAT (eWAT) because of the upregulation of genes promoting lipolytic activity. Expression of microRNA 145 (miR-145) is decreased because of impaired primary miR-145 processing in Ksrp-/- eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through posttranscriptional regulation of miR-145 expression.
|Titolo:||KSRP and MicroRNA 145 are negative regulators of lipolysis in white adipose tissue|
|Parole Chiave:||Diet-induced obesity; hormone-sensitive lipase; RNA-binding protein; triglyceride lipase; messenger-RNA; adipocytes; promotes; ablation; overexpression; biogenesis|
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
|Data di pubblicazione:||2014|
|Digital Object Identifier (DOI):||10.1128/MCB.00042-14|
|Appare nelle tipologie:||01 - Articolo su periodico|