Eukaryotic DNA replication initiation and the Fanconi anemia pathway of interstrand crosslink repair both revolve around the recruitment of a set of DNA-processing factors onto a dimeric protein complex, which functions as a loading platform (MCM and FANCI-FANCD2 respectively). Here we compare and contrast the two systems, identifying a set of unresolved mechanistic questions. How is the dimeric loading platform assembled on the DNA? How can equivalent covalent modification of both factors in a dimer be achieved? Are multicomponent DNA-interacting machines built symmetrically around their dimeric loading platform? Recent biochemical reconstitution studies are starting to shed light on these issues.

DNA replication and inter-strand crosslink repair : symmetric activation of dimeric nanomachines? / P. Swuec, A. Costa. - In: BIOPHYSICAL CHEMISTRY. - ISSN 0301-4622. - 225(2017), pp. 15-19.

DNA replication and inter-strand crosslink repair : symmetric activation of dimeric nanomachines?

P. Swuec
Primo
;
2017

Abstract

Eukaryotic DNA replication initiation and the Fanconi anemia pathway of interstrand crosslink repair both revolve around the recruitment of a set of DNA-processing factors onto a dimeric protein complex, which functions as a loading platform (MCM and FANCI-FANCD2 respectively). Here we compare and contrast the two systems, identifying a set of unresolved mechanistic questions. How is the dimeric loading platform assembled on the DNA? How can equivalent covalent modification of both factors in a dimer be achieved? Are multicomponent DNA-interacting machines built symmetrically around their dimeric loading platform? Recent biochemical reconstitution studies are starting to shed light on these issues.
Animals; DNA; Dimerization; Fanconi Anemia; Fanconi Anemia Complementation Group D2 Protein; Humans; DNA Repair; DNA Replication; Biophysics; Biochemistry; Organic Chemistry
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/618940
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