Monoubiquitination and deubiquitination of FANCD2:FANCI heterodimer is central to DNA repair in a pathway that is defective in the cancer predisposition syndrome Fanconi anemia (FA). The “FA core complex” contains the RING-E3 ligase FANCL and seven other essential proteins that are mutated in various FA subtypes. Here, we purified recombinant FA core complex to reveal the function of these other proteins. The complex contains two spatially separate FANCL molecules that are dimerized by FANCB and FAAP100. FANCC and FANCE act as substrate receptors and restrict monoubiquitination to the FANCD2:FANCI heterodimer in only a DNA-bound form. FANCA and FANCG are dispensable for maximal in vitro ubiquitination. Finally, we show that the reversal of this reaction by the USP1:UAF1 deubiquitinase only occurs when DNA is disengaged. Our work reveals the mechanistic basis for temporal and spatial control of FANCD2:FANCI monoubiquitination that is critical for chemotherapy responses and prevention of Fanconi anemia.

Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway / S. van Twest, V.J. Murphy, C. Hodson, W. Tan, P. Swuec, J.J. O'Rourke, J. Heierhorst, W. Crismani, A.J. Deans. - In: MOLECULAR CELL. - ISSN 1097-2765. - 65:2(2017 Jan), pp. 247-259.

Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway

P. Swuec;
2017-01

Abstract

Monoubiquitination and deubiquitination of FANCD2:FANCI heterodimer is central to DNA repair in a pathway that is defective in the cancer predisposition syndrome Fanconi anemia (FA). The “FA core complex” contains the RING-E3 ligase FANCL and seven other essential proteins that are mutated in various FA subtypes. Here, we purified recombinant FA core complex to reveal the function of these other proteins. The complex contains two spatially separate FANCL molecules that are dimerized by FANCB and FAAP100. FANCC and FANCE act as substrate receptors and restrict monoubiquitination to the FANCD2:FANCI heterodimer in only a DNA-bound form. FANCA and FANCG are dispensable for maximal in vitro ubiquitination. Finally, we show that the reversal of this reaction by the USP1:UAF1 deubiquitinase only occurs when DNA is disengaged. Our work reveals the mechanistic basis for temporal and spatial control of FANCD2:FANCI monoubiquitination that is critical for chemotherapy responses and prevention of Fanconi anemia.
core complex; deubiquitination; DNA repair; enzyme mechanism; FANCB; FANCD2; Fanconi anemia; monoubiquitination; RING E3; Cell Line; DNA; DNA-Binding Proteins; Fanconi Anemia; Fanconi Anemia Complementation Group A Protein; Fanconi Anemia Complementation Group C Protein; Fanconi Anemia Complementation Group D2 Protein; Fanconi Anemia Complementation Group E Protein; Fanconi Anemia Complementation Group G Protein; Fanconi Anemia Complementation Group L Protein; Fanconi Anemia Complementation Group Proteins; Humans; Inhibitor of Differentiation Protein 2; Multiprotein Complexes; Nuclear Proteins; Protein Binding; Protein Multimerization; Recombinant Proteins; Substrate Specificity; Time Factors; Transfection; Ubiquitin-Specific Proteases; Ubiquitination; Molecular Biology; Cell Biology
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/618936
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