Background: Early steps of HIV infection are mediated by the binding of the envelope to mucosal receptors as α4β7 and the C-type lectins DC-SIGN and langerin. Previously Env-specific B-cell responses have been reported in highly exposed seronegative individuals (HESN). Method: Here, we studied gp120-specific antibodies ability to block HIV interaction with α4β7, DC-SIGN and/or langerinin HESN. New cell-based assays were developed to analyze whether antibodies that can alter gp120 binding to α4β7, DC-SIGN and/or langerin are induced in HESN. A mucosal blocking score (MBS) was defined based on the ability of antibodies to interfere with gp120/α4β7, gp120/DC-SIGN, and gp120/langerin binding. A new MBS was evaluated in a cohort of 86 HESN individuals and compared with HIV+ patients or HIV− unexposed healthy individuals. Results: Antibodies reducing gp120 binding to both α4β7 and DC-SIGN were present in HESN serum but also in mucosal secretions, whereas antibodies from HIV+ patients facilitated gp120 binding to DC-SIGN. Any correlation was observed between MBS and the capacity of antibodies to neutralize infection of α4β7+ CD4+ T cells with primary isolates. Conclusions: MBS is significantly associated with protection in HESN and might reflect altered HIV spreading to mucosal-associated lymphoid tissues.
A high mucosal blocking score is associated with HIV protection / A. Girard, N. Rallón, J.M. Benito, F. Jospin, C. Rodriguez, B. Chanut, F. Benjelloun, J. Del Romero, B. Verrier, F. Lucht, J. Pin, C. Genin, M. Biasin, M. Clerici, S. Paul. - In: AIDS. - ISSN 0269-9370. - 33:3(2019 Mar 01), pp. 411-423.
|Titolo:||A high mucosal blocking score is associated with HIV protection|
CLERICI, MARIO SALVATORE (Penultimo)
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
Settore MED/04 - Patologia Generale
|Data di pubblicazione:||1-mar-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1097/QAD.0000000000002099|
|Appare nelle tipologie:||01 - Articolo su periodico|
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