Human Herpes Simplex Virus type 1 (HSV-1) infection is suggested to play a role in the development of Alzheimer's disease (AD). Immunoglobulin G (IgG) neutralize HSV-1 activity, but the virus can evade IgG-mediated immune responses by expressing receptor that efficiently binds the Fc portion of all IgG subclasses with the exception of IgG3. We analyzed HSV-1-specific IgG subclasses and IgG-mediated serum neutralization activity against HSV-1 in individuals with a diagnosis of either AD or mild cognitive impairment (MCI), comparing the results with those obtained in age-matched healthy controls (HC). 186 individuals were enrolled in the study: 67 AD, 58 MCI, and 61 HC. HSV-1 IgG titers and subclasses, neutralizing antibody (NAb) titers, and complement C3 concentration-critical component of antibody-mediated effector activity-were measured in sera by ELISA; IgG neutralizing activity was performed on HSV-1 infected Vero cells. Results showed that, whereas HSV-1-specific IgG1, IgG2, and IgG4 titers as well as complement C3 serum concentration were comparable in all groups of individuals, IgG3 were more frequently detected in MCI (89%) compared to AD (75%; p < 0.05) and HC (68%; p = 0.003), whereas the titer is similar among the three groups (AD: 0.66 ± 0.21 OD; MCI: 0.68 ± 0.24 OD; HC: 0.72 ± 0.28 OD). Notably, HSV-1 specific neutralizing ability of AD sera was reduced even in the presence of high quantity of IgG3. As IgG3 plays a key role in counteracting the ability of HSV-1 to evade immune responses, these data reinforce the hypothesis of a pathogenetic role of HSV-1 in AD.
HSV-1-Specific IgG Subclasses Distribution and Serum Neutralizing Activity in Alzheimer's Disease and in Mild Cognitive Impairment / S. Agostini, R. Mancuso, A. Hernis, A.S. Costa, R. Nemni, M. Clerici. - In: JOURNAL OF ALZHEIMER'S DISEASE. - ISSN 1387-2877. - 63:1(2018), pp. 131-138.
|Titolo:||HSV-1-Specific IgG Subclasses Distribution and Serum Neutralizing Activity in Alzheimer's Disease and in Mild Cognitive Impairment|
AGOSTINI, SIMONE (Corresponding)
|Parole Chiave:||Alzheimer's disease; HSV-1; HSV-1-IgG subclasses; Mild cognitive impairment; Neutralization activity; Neuroscience (all); Clinical Psychology; Geriatrics and Gerontology; Psychiatry and Mental Health|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore MED/26 - Neurologia
|Data di pubblicazione:||2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3233/JAD-170966|
|Appare nelle tipologie:||01 - Articolo su periodico|